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Molecule Parameter List for GTP.Ran-nuclear

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

GTP.Ran-nuclear participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
RanGTPase	70	Network	Shared_Object_RanGTPase,  Nucleus,  Cytoplasm
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

GTP.Ran-nuclear acting as a Molecule in  RanGTPase Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
GTP.Ran-nuclear	RanGTPase Accession No. : 70	Nucleus Pathway No. : 305	0	12000	No
nuclear-GTP.Ran Nuclear fraction of Ran-GTP complex Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2 Gorlich D et al (2003) EMBO J 22(5):1088-1100

GTP.Ran-nuclear acting as a Substrate in a reaction in  RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Cytoplasmic_ transfer	RanGTPase Accession No. : 70	Shared_Object_ RanGTPase Pathway No. : 304	0.08 (s^-1)	1.3 (s^-1)	Not applicable**	-	Substrate GTP.Ran-nuclear Product GTP.Ran-cytoplas mic
Approximation to achieve Cytoplasmic transfer as per kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100

** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration

GTP.Ran-nuclear acting as a Product in a reaction in  RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
GTP.Ran_release	RanGTPase Accession No. : 70	Nucleus Pathway No. : 305	55 (s^-1)	99.9994 (uM^-1 s^-1)	Kd(cb) = 1.8182(uM)	-	Substrate RCC1-RanGTP Product GTP.Ran-nuclear RCC1
Release of GTP.Ran from GTP.Ran-RCC1 complex Kf = 55 /sec Kb = 1.0e+08 /M/sec = 1.0e+02 /uM/sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100