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Molecule Parameter List for Raf-GTP-Ras | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2004_PKM_Tuning | 76 | Network |
PKC, Shared_Object_Ajay_Bhalla_2004_PKM_tuning, PLA2,
PLCbeta, Gq, MAPK,
Ras, EGFR, Sos,
PLC_g, CaMKII, CaM,
PP1, PP2B, PKA,
AC, PKM | | This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a. |
Raf-GTP-Ras acting as a Molecule in Ajay_Bhalla_2004_PKM_Tuning Network
Raf-GTP-Ras acting as an Enzyme in Ajay_Bhalla_2004_PKM_Tuning Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | Raf-GTP-Ras /
Raf-GTP-Ras.1
| Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK
Product
MAPKK-ser
| | | Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). | | 2 | Raf-GTP-Ras /
Raf-GTP-Ras.2
| Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | MAPK
Pathway No. : 317 | 0.159094 | 0.3 | 4 | explicit E-S complex | Substrate
MAPKK-ser
Product
MAPKK*
| | | Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591). |
Raf-GTP-Ras acting as a Product in a reaction in Ajay_Bhalla_2004_PKM_Tuning Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | Ras-act-unphosph
-raf | Ajay_Bhalla_
2004_PKM_Tuning
Accession No. : 76 | Shared_Object_
Ajay_Bhalla_
2004_PKM_tuning
Pathway No. : 312 | 0
(uM^-1 s^-1) | 0
(s^-1) | - | - | Substrate
GTP-Ras
craf-1
Product
Raf-GTP-Ras
| | 18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
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