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Molecule Parameter List for PKC-DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PKC-DAG* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	1	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
3d_fold_model	8	Network	Shared_Object_3d_fold_model, PKC, MAPK, PLA2, Ras
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

PKC-DAG acting as a Molecule in 3d_fold_model Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PKC-DAG	3d_fold_model Accession No. : 8	PKC Pathway No. : 55	0	1000	No
CoInit was .0624

PKC-DAG acting as a Substrate in a reaction in 3d_fold_model Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PKC-n-DAG-AA	3d_fold_model Accession No. : 8	PKC Pathway No. : 55	0.018 (uM^-1 s^-1)	2 (s^-1)	Kd(bf) = 111.1111(uM)	-	Substrate AA PKC-DAG Product PKC-DAG-AA
Reduced kf to 0.66X to match Shinomura et al data. Initial: kf = 3.3333e-9 New: 2e-9 Newer: 2e-8 kb was 0.2 now 2.

PKC-DAG acting as a Product in a reaction in 3d_fold_model Network

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PKC-n-DAG	3d_fold_model Accession No. : 8	PKC Pathway No. : 55	0.0006 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 166.6667(uM)	-	Substrate DAG PKC-cytosolic Product PKC-DAG
kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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