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Molecule Parameter List for MAPKK

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MAPKK* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	25	0	0	50	25	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13	82	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff1_1e-13, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, kinetics[3], PKC, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, kinetics[6], CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, MAPK, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM
This is a 25-compartment reaction-diffusion version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 25 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec. For D = 1e-13 m^2/sec (i.e., 0.1 micron^2/sec ) the kf and kb are 0.001/sec. The stimulus file pkm_mapk22_diff_1e-13_Fig4B which was used for the model to replicate Figure 4B from the paper. pkm_mapk22_diff_1e-13_Fig4H replicate Figure 4H. pkm_mapk22_diff_1e-13_Fig4I replicate Figure 4I.

MAPKK acting as a Molecule in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 528	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 535	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 541	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 547	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 553	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 559	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 565	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 571	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 577	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 583	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 589	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 595	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 601	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 607	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 613	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 619	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 625	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 631	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 637	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 643	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 649	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 655	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 661	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 667	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.
MAPKK	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 673	0.5	1.5	No
Conc is from Seger et al JBC 267:20 pp14373 (1992) mwt is 45/46 Kd We assume that phosphorylation on both ser and thr is needed for activiation. See Kyriakis et al Nature 358 417 1992 Init conc of total is 0.18 Ortiz et al 1995 J Neurosci 15(2):1285-1297 suggest that levels are higher in hippocampus than other brain regions, and further elevated in synapses. Estimate 3x higher levels than before, at 0.5 uM. Similar results from Schipper et al 1999 Neuroscience 93(2):585-595 but again lacking in quantitation.

MAPKK acting as a Substrate for an Enzyme in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 528	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 528	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
3	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 535	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
4	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 535	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
5	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 541	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
6	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 541	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
7	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 547	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
8	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 547	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
9	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 553	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
10	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 553	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
11	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 559	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
12	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 559	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
13	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 565	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
14	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 565	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
15	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 571	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
16	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 571	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
17	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 577	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
18	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 577	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
19	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 583	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
20	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 583	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
21	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 589	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
22	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 589	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
23	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 595	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
24	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 595	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
25	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 601	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
26	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 601	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
27	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 607	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
28	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 607	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
29	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 613	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
30	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 613	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
31	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 619	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
32	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 619	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
33	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 625	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
34	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 625	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
35	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 631	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
36	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 631	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
37	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 637	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
38	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 637	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
39	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 643	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
40	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 643	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
41	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 649	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
42	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 649	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
43	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 655	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
44	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 655	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
45	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 661	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
46	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 661	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
47	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 667	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
48	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 667	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
49	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 673	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
50	Raf-GTP-Ras / Raf-GTP-Ras.1	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	MAPK Pathway No. : 673	0.159096	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6

MAPKK acting as a Product of an Enzyme in Ajay_Bhalla_2007_ReacDiff1_1e-13 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Pathway No. : 526	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

2	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics Pathway No. : 533	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

3	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[1] Pathway No. : 539	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

4	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[2] Pathway No. : 545	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

5	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[3] Pathway No. : 551	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

6	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[4] Pathway No. : 557	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

7	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[5] Pathway No. : 563	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

8	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[6] Pathway No. : 567	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

9	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[7] Pathway No. : 575	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

10	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[8] Pathway No. : 581	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

11	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[9] Pathway No. : 587	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

12	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[10] Pathway No. : 593	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

13	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[11] Pathway No. : 599	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

14	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[12] Pathway No. : 605	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

15	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[13] Pathway No. : 611	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

16	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[14] Pathway No. : 617	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

17	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[15] Pathway No. : 623	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

18	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[16] Pathway No. : 629	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

19	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[17] Pathway No. : 635	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

20	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[18] Pathway No. : 641	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

21	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[19] Pathway No. : 647	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

22	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[20] Pathway No. : 653	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

23	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[21] Pathway No. : 659	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

24	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[22] Pathway No. : 665	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

25	PPhosphatase2A / MAPKK-deph-ser	Ajay_Bhalla_ 2007_ReacDiff1_ 1e-13 Accession No. : 82	kinetics[23] Pathway No. : 671	15.6567	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

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