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Molecule Parameter List for MKP-1**

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

MKP-1** participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	0	1	2	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
MAPK_MKP1_ oscillation	9	Network	Shared_Object_MAPK_MKP1_oscillation,  PKC,  MAPK,   PLA2,  Ras
This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

MKP-1** acting as a Molecule in  MAPK_MKP1_oscillation Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MKP-1**	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0	1000	No
3 Feb 2000. Same enz activity as MKP-1, but different degradation rates. Brondello et al Science 286:2514 1999 4 Feb. Actually this form is doubly phosphorylated.

MKP-1** acting as an Enzyme in  MAPK_MKP1_oscillation Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MKP-1** / MKP1*-tyr-deph	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0.0666667	1	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MAPK-tyr Product MAPK
	3 Feb 2000. Same rates as MKP-1.
2	MKP-1** / MKP1*-thr-deph	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0.0666667	1	4	Classical Michaelis-Menten V = Etot.S.Kcat/Km+S	Substrate MAPK* Product MAPK-tyr
	3 Feb 2000. Same rates as MKP1

MKP-1** acting as a Product of an Enzyme in  MAPK_MKP1_oscillation Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
MAPK*  / MKP-1-phosph2	MAPK_MKP1_ oscillation Accession No. : 9	Shared_Object_ MAPK_MKP1_ oscillation Pathway No. : 59	25.641	1	4	explicit E-S complex	Substrate MKP-1-ser359* Product MKP-1**
3 Feb 2000. See Brondello et al Science 286:2514 1999. Rates assumed standard MAPK rates based on Sanghera et al JBC 265(1):53-57 1990. 24 Apr 2K, based on 12 Feb 2K: Scaled x0.1 to 3.25e-7,4,1

MKP-1** acting as a Substrate in a reaction in  MAPK_MKP1_oscillation Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	turnover_MKP1**	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0.0001 (s^-1)	0 (s^-1)	-	-	Substrate MKP-1** Product Ubiquitination
	3 Feb 2000. Rate of turnover of phosph form of MKP1 is from Brondello et al Science 286:2514 1999. Tau is about 150 min though it is not a clean exp falloff.
2	MKP-1**dephosph	MAPK_MKP1_ oscillation Accession No. : 9	MAPK Pathway No. : 61	0.01 (s^-1)	0 (s^-1)	-	-	Substrate MKP-1** Product MKP-1-ser359*
	24 Apr 2K, based on 12 Feb 2K: Scaled up 10x to kf=0.01, kb=0