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Molecule Parameter List for DAG

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
DAG participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000030

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • MAPK_MKP1_
    oscillation
    		• 9Network
    Shared_Object_MAPK_MKP1_oscillation,  PKC,  MAPK,  
    PLA2,  Ras 
    This model relates to figure 5 in Bhalla US, Iyengar R. Chaos (2001) 11(1):221-226. It includes the model used for figures 2-4 and also has MKP-1 induction by MAPK activity in the synapse. PP2A is set to 0.16 uM and MKP synthesis is varied from 5x to 40 x basal to get a range of interesting behaviours.

    DAG acting as a Molecule in      MAPK_MKP1_oscillation Network
    NameAccession NamePathway NameInitial Conc.
    (uM)    		Volume
    (fL)    		Buffered
    DAG
  • MAPK_MKP1_
    oscillation
    Accession No. : 9
  • Shared_Object_
    MAPK_MKP1_
    oscillation
    Pathway No. : 59
    		• 11.6611000Yes
    Baseline is 11.661 from running combined model to steady state. See ALL/NOTES for 23 Apr 1998.

    DAG acting as a Substrate in a reaction in      MAPK_MKP1_oscillation Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1PKC-act-by-DAG
  • MAPK_MKP1_
    oscillation
    Accession No. : 9
    		• PKC
    Pathway No. : 60    		0.008
    (uM^-1 s^-1)    		8.6348
    (s^-1)    		Kd(bf) = 1079.377(uM)-Substrate
    DAG
    PKC-Ca

    Product
    PKC-Ca-DAG
      Need est of rate. Assume it is fast Obtained from param search kf raised 10 X : see Shinomura et al PNAS 88 5149-5153 1991. kf changed from 3.865e-7 to 2.0e-7 in line with closer analysis of Shinomura data. 26 June 1996: Corrected DAG data: reduce kf 15x from 2e-7 to 1.333e-8
    2PKC-n-DAG
  • MAPK_MKP1_
    oscillation
    Accession No. : 9
    		• PKC
    Pathway No. : 60    		0.0006
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 166.6667(uM)-Substrate
    DAG
    PKC-cytosolic

    Product
    PKC-DAG
      kf raised 10 X based on Shinomura et al PNAS 88 5149-5153 1991 closer analysis of Shinomura et al: kf now 1e-8 (was 1.66e-8). Further tweak. To get sufficient AA synergy, increase kf to 1.5e-8 26 June 1996: Corrected DAG levels: reduce kf by 15x from 1.5e-8 to 1e-9
    3DAG-Ca-PLA2-act
  • MAPK_MKP1_
    oscillation
    Accession No. : 9
    		• PLA2
    Pathway No. : 62    		0.003
    (uM^-1 s^-1)    		4
    (s^-1)    		Kd(bf) = 1333.3333(uM)-Substrate
    DAG
    PLA2-Ca*

    Product
    DAG-Ca-PLA2*
      27 June 1996 Scaled kf down by 0.015 from 3.33e-7 to 5e-9 to fit with revised DAG estimates and use of mole-fraction to calculate eff on PLA2.


    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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