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Molecule Parameter List for MAPKK-ser

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

MAPKK-ser participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	2	2	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
mTOR_pathway	92	Network	Shared_Object_mTOR_pathway,  AKT_mod,  S6Kinase,   PI3K_mod,  TrKB_mod,  mTOR_model,   MAPK,  PKC,  4E-BP_mod,   Ras,  Sos,  43S_complex,   CaM
This model consists of various sub-modules. They are as follows: 1) BDNF receptor signaling 2) AKT signaling 3) 4E-BP model 4) S6 Kinase model 5) CaMKIII model 6) Protein synthesis model 7) CaM 8) PKC 9) MAPK model.

MAPKK-ser acting as a Molecule in  mTOR_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK-ser	mTOR_pathway Accession No. : 92	MAPK Pathway No. : 1103	0	1000	No
Intermediately phophorylated, assumed inactive, form of MAPKK

MAPKK-ser acting as a Substrate for an Enzyme in  mTOR_pathway Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf_ star-GTP-Ras  / Raf_ star-GTP-Ras.2	mTOR_pathway Accession No. : 92	MAPK Pathway No. : 1103	0.159094	0.3	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK_star
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
2	PPhosphatase2A  / MAPKK-deph-ser	mTOR_pathway Accession No. : 92	Shared_Object_ mTOR_pathway Pathway No. : 1097	15.6568	6	4	explicit E-S complex	Substrate MAPKK-ser Product MAPKK

MAPKK-ser acting as a Product of an Enzyme in  mTOR_pathway Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf_ star-GTP-Ras  / Raf_ star-GTP-Ras.1	mTOR_pathway Accession No. : 92	MAPK Pathway No. : 1103	0.159094	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK-ser
	Kinetics are the same as for the craf-1_star activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
2	PPhosphatase2A  / MAPKK-deph	mTOR_pathway Accession No. : 92	Shared_Object_ mTOR_pathway Pathway No. : 1097	15.6568	6	4	explicit E-S complex	Substrate MAPKK_star Product MAPKK-ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.