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Molecule Parameter List for MAPKK_dash_ser

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MAPKK_dash_ser* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	5	5	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
mRNA synthesis	94	Network	kinetics, compartment_1, compartment_2
The model consists of three major pathways: Calcium-calmodulin dependent protein kinase IV (CaMKIV), Mitogen-activated protein kinase (MAPK) and Protein Phosphatase 1 (PP1). Each of these converged on CREB activation. We also modeled further interactions with Transducer of regulated CREB activity 1 (TORC1) and the protein kinase A (PKA) pathway.

MAPKK_dash_ser acting as a Molecule in mRNA synthesis Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK_dash_ser	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0	1000	No
Intermediately phophorylated, assumed inactive, form of MAPKK

MAPKK_dash_ser acting as a Substrate for an Enzyme in mRNA synthesis Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	bRaf_Rap1GTP / braf_dash_Rap1_ dash_GTP2	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.16	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star

2	Raf_dash_GTP_ dash_Ras / Raf_dash_GTP_ dash_Ras.2	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
3	braf_dash_GTP_ dash_Ras / braf_dash_GTP_ dash_Ras2	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.16	0.2	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star

4	Raf_star_dash_ GTP_dash_Ras / Raf_star_dash_ GTP_dash_Ras.2	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6
5	PPhosphatase2A / MAPKK_dash_ deph_dash_ser	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	15.6568	6	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK

MAPKK_dash_ser acting as a Product of an Enzyme in mRNA synthesis Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	bRaf_Rap1GTP / braf_dash_rap1_ dash_GTP1	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.16	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK_dash_ser

2	Raf_dash_GTP_ dash_Ras / Raf_dash_GTP_ dash_Ras.1	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK_dash_ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
3	braf_dash_GTP_ dash_Ras / braf_dash_GTP_ dash_Ras1	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.16	0.2	4	explicit E-S complex	Substrate MAPKK Product MAPKK_dash_ser

4	Raf_star_dash_ GTP_dash_Ras / Raf_star_dash_ GTP_dash_Ras.1	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK_dash_ser
	Kinetics are the same as for the craf-1* activity, ie., k1=1.1e-6, k2=.42, k3 =0.105 These are based on Force et al PNAS USA 91 1270-1274 1994. These parms cannot reach the observed 4X stim of MAPK. So lets increase the affinity, ie, raise k1 10X to 1.1e-5 Lets take it back down to where it was. Back up to 5X: 5.5e-6
5	PPhosphatase2A / MAPKK_dash_deph	mRNA synthesis Accession No. : 94	kinetics Pathway No. : 1112	15.6568	6	4	explicit E-S complex	Substrate MAPKK_star Product MAPKK_dash_ser
	See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

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