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Molecule Parameter List for MAPKK_star

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
MAPKK_star participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1021400

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
mRNA synthesis94Network
kinetics compartment_1 compartment_2 
The model consists of three major pathways: Calcium-calmodulin dependent protein kinase IV (CaMKIV), Mitogen-activated protein kinase (MAPK) and Protein Phosphatase 1 (PP1). Each of these converged on CREB activation. We also modeled further interactions with Transducer of regulated CREB activity 1 (TORC1) and the protein kinase A (PKA) pathway.

MAPKK_star acting as a Molecule in  
mRNA synthesis Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
MAPKK_starmRNA synthesis
Accession No. : 94
kinetics
Pathway No. : 1112
01000No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992

MAPKK_star acting as an Enzyme in  
mRNA synthesis Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1MAPKK_star /
MAPKKtyr
mRNA synthesis
Accession No. : 94
kinetics
Pathway No. : 1112
0.04630.34explicit E-S complexSubstrate
MAPK

Product
MAPK_dash_tyr
    The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2MAPKK_star /
MAPKKthr
mRNA synthesis
Accession No. : 94
kinetics
Pathway No. : 1112
0.04630.34explicit E-S complexSubstrate
MAPK_dash_tyr

Product
MAPK_star
    Rate consts same as for MAPKKtyr.

MAPKK_star acting as a Substrate for an Enzyme in  
mRNA synthesis Network
Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
PPhosphatase2A  /
MAPKK_dash_deph
mRNA synthesis
Accession No. : 94
kinetics
Pathway No. : 1112
15.656864explicit E-S complexSubstrate
MAPKK_star

Product
MAPKK_dash_ser
See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

MAPKK_star acting as a Product of an Enzyme in  
mRNA synthesis Network
 Enzyme Molecule /
Enzyme Activity
Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
1bRaf_Rap1GTP  /
  • braf_dash_Rap1_
    dash_GTP2
  • mRNA synthesis
    Accession No. : 94
    kinetics
    Pathway No. : 1112
    0.160.34explicit E-S complexSubstrate
    MAPKK_dash_ser

    Product
    MAPKK_star
       
    2
  • Raf_dash_GTP_
    dash_Ras
      /
  • Raf_dash_GTP_
    dash_Ras.2
  • mRNA synthesis
    Accession No. : 94
    kinetics
    Pathway No. : 1112
    0.15910.34explicit E-S complexSubstrate
    MAPKK_dash_ser

    Product
    MAPKK_star
        Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
    3
  • braf_dash_GTP_
    dash_Ras
      /
  • braf_dash_GTP_
    dash_Ras2
  • mRNA synthesis
    Accession No. : 94
    kinetics
    Pathway No. : 1112
    0.160.24explicit E-S complexSubstrate
    MAPKK_dash_ser

    Product
    MAPKK_star
       
    4
  • Raf_star_dash_
    GTP_dash_Ras
      /
  • Raf_star_dash_
    GTP_dash_Ras.2
  • mRNA synthesis
    Accession No. : 94
    kinetics
    Pathway No. : 1112
    0.15910.34explicit E-S complexSubstrate
    MAPKK_dash_ser

    Product
    MAPKK_star
        Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6



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