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Molecule Parameter List for Raf_dash_GTP_dash_Ras

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

Raf_dash_GTP_dash_Ras participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Differential syn thesis of mRNA	95	Network	kinetics,  compartment_1,  compartment_2
The model consists of three major pathways: Calcium-calmodulin dependent protein kinase IV (CaMKIV), Mitogen-activated protein kinase (MAPK) and Protein Phosphatase 1 (PP1). Each of these converged on CREB activation. We also modeled further interactions with Transducer of regulated CREB activity 1 (TORC1) and the protein kinase A (PKA) pathway.

Raf_dash_GTP_dash_Ras acting as a Molecule in  Differential synthesis of mRNA Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
Raf_dash_GTP_dash_Ras	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0	1000	No

Raf_dash_GTP_dash_Ras acting as an Enzyme in  Differential synthesis of mRNA Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	Raf_dash_GTP_ dash_Ras / Raf_dash_GTP_ dash_Ras.1	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK Product MAPKK_dash_ser
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
2	Raf_dash_GTP_ dash_Ras / Raf_dash_GTP_ dash_Ras.2	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).

Raf_dash_GTP_dash_Ras acting as a Product in a reaction in  Differential synthesis of mRNA Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
Ras_dash_act_ dash_unphosph_ dash_raf	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	6 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.1667(uM)	-	Substrate GTP_dash_Ras craf_dash_1 Product Raf_dash_GTP_ dash_Ras
18 May 2003. This reaction is here to provide basal activity for MAPK as well as the potential for direct EGF stimulus without PKC activation. Based on model from FB/fb28c.g: the model used for MKP-1 turnover. The rates there were constrained by basal activity values.