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Molecule Parameter List for MAPKK_star

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*MAPKK_star* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	1	4	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Differential syn thesis of mRNA	95	Network	kinetics, compartment_1, compartment_2
The model consists of three major pathways: Calcium-calmodulin dependent protein kinase IV (CaMKIV), Mitogen-activated protein kinase (MAPK) and Protein Phosphatase 1 (PP1). Each of these converged on CREB activation. We also modeled further interactions with Transducer of regulated CREB activity 1 (TORC1) and the protein kinase A (PKA) pathway.

MAPKK_star acting as a Molecule in Differential synthesis of mRNA Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
MAPKK_star	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0	1000	No
MAPKK phosphorylates MAPK on both the tyr and thr residues, first tyr then thr. Refs: Seger et al JBC267:20 pp 14373 1992 The MAPKK itself is phosphorylated on ser as well as thr residues. Let us assume that the ser goes first, and that the sequential phosphorylation is needed. See Kyriakis et al Nature 358 417-421 1992

MAPKK_star acting as an Enzyme in Differential synthesis of mRNA Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	MAPKK_star / MAPKKtyr	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.0463	0.3	4	explicit E-S complex	Substrate MAPK Product MAPK_dash_tyr
	The actual MAPKK is 2 forms from Seger et al JBC 267:20 14373(1992) Vmax = 150nmol/min/mg From Haystead et al FEBS 306(1):17-22 we get Km=46.6nM for at least one of the phosphs. Putting these together: k3=0.15/sec, scale to get k2=0.6. k1=0.75/46.6nM=2.7e-5
2	MAPKK_star / MAPKKthr	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.0463	0.3	4	explicit E-S complex	Substrate MAPK_dash_tyr Product MAPK_star
	Rate consts same as for MAPKKtyr.

MAPKK_star acting as a Substrate for an Enzyme in Differential synthesis of mRNA Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PPhosphatase2A / MAPKK_dash_deph	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	15.6568	6	4	explicit E-S complex	Substrate MAPKK_star Product MAPKK_dash_ser
See: Kyriakis et al Nature 358 pp 417-421 1992 Ahn et al Curr Op Cell Biol 4:992-999 1992 for this pathway. See parent PPhosphatase2A for parms.

MAPKK_star acting as a Product of an Enzyme in Differential synthesis of mRNA Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	bRaf_Rap1GTP / braf_dash_Rap1_ dash_GTP2	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.16	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star

2	Raf_dash_GTP_ dash_Ras / Raf_dash_GTP_ dash_Ras.2	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star
	Kinetics are the same as for the craf_1* activity, ie., k1=5.5e-6, k2=0.42, k3 = 0.105 These are basedo n Force et al PNAS USA 91 1270-1274, 1994., but k1 is scaled up 5x (ie., Km is scaled down 5x to the value used here and for craf_1* activity: Km = 0.1591).
3	braf_dash_GTP_ dash_Ras / braf_dash_GTP_ dash_Ras2	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.16	0.2	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star

4	Raf_star_dash_ GTP_dash_Ras / Raf_star_dash_ GTP_dash_Ras.2	Differential syn thesis of mRNA Accession No. : 95	kinetics Pathway No. : 1115	0.1591	0.3	4	explicit E-S complex	Substrate MAPKK_dash_ser Product MAPKK_star
	Same kinetics as other c-raf activated forms. See Force et al PNAS 91 1270-1274 1994. k1 = 1.1e-6, k2 = .42, k3 = 1.05 raise k1 to 5.5e-6

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