Enter a Search String |
| Special character and space not allowed in the query term. Search string should be at least 2 characters long. |
Enzyme List for pathway PDGFR (Pathway Number 38) in Accession mkp1_feedback_effects (Accession Number 4) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.Further ordering can be done according to header.   arrow indicates that ordering is done according to ascending or descending order. |
| Enzyme Molecule / Enzyme Activity | Pathway Name / Pathway No. | Km (uM) | kcat (s^-1) | Ratio (k2/k3) | Enzyme Type | Reagents | ||
| 1 | L.PDGFR / phosph_Shc | PDGFR Pathway No. 38 | 0.833333 | 0.05 | 4 | explicit E-S complex | Substrate: SHC Product : SHC* | |
| Rates from Okada et al JBC 270:35 pp 20737 1995 Km = 0.70 to 0.85 uM, Vmax = 4.4 to 5.0 pmol/min. Unfortunately the amount of enzyme is not known, the prep is only partially purified. Tau phosph is max within 30 sec, falls back within 20 min. Ref: Sasaoka et al JBC 269:51 32621 1994. Use k3 = 0.1 based on this tau. 27 Apr 2001: Lowered k3 to 0.05 to fix conc-effect of SHC phosph by PDGF. This gives results for downstream effects in agreement with other papers, e.g., the Brondello papers. | ||||||||