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Reaction List for pathway PKA (Pathway Number 196) in Accession PKA_2003 (Accession Number 47) |
Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.   indicates that ordering is done according to ascending or descending order.Keq is calculated only for first order reactions. Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules] |
| Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents | |
| 1 | 1 | PKA Pathway No. 196 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: R2C2 cAMP Products: cAMP.R2C2 | Hasler et al (1992 jun) FASEB J. 6(9):2735-41 say Kd =1e-7M for type II, 5.6e-8 M for type I. Take mean which comes to 2e-13 #/cell Smith et al (1981 Mar) Proc Natl Acad Sci U S A 78(3) 1591-1595 have better data. First kf/kb=2.1e7/M = 3.5e-5 (#/cell).Ogreid and Doskeland (1981 Jul 6) FEBS Lett 129(2) 287-292 have figs suggesting time course of complete assoc is < 1 min. |
| 2 | 2 | PKA Pathway No. 196 | 54 (uM^-1 s^-1) | 33 (s^-1) | Kd(bf) = 0.6111(uM) | - | Substrate: cAMP.R2C2 cAMP Products: cAMP2.R2C2 | For now let us set this to the same Km (1e-7M) as site B. This gives kf/kb = .7e-7M * 1e6 / (6e5^2) : 1/(6e5^2) = 2e-13:2.77e-12 Smith et al (1981 Mar) Proc Natl Acad Sci U S A 78(3) 1591-1595 have better values. They say that this is cooperative, so the consts are now kf/kb =8.3e-4 |
| 3 | 1 | PKA Pathway No. 196 | 75 (uM^-1 s^-1) | 110 (s^-1) | Kd(bf) = 1.4667(uM) | - | Substrate: cAMP2.R2C2 cAMP Products: cAMP3.R2C2 |
| 4 | 2 | PKA Pathway No. 196 | 75 (uM^-1 s^-1) | 32.5 (s^-1) | Kd(bf) = 0.4333(uM) | - | Substrate: cAMP cAMP3.R2C2 Products: cAMP4.R2C2 |
| 5 | Release-C1 | PKA Pathway No. 196 | 60 (s^-1) | 18 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: cAMP4.R2C2 Products: PKA-active cAMP4.R2C | This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10 |
| 6 | Release-C2 | PKA Pathway No. 196 | 60 (s^-1) | 18 (uM^-1 s^-1) | Kd(cb) = 0.3(uM) | - | Substrate: cAMP4.R2C Products: PKA-active cAMP4.R2 |
| 7 | inhib-PKA | PKA Pathway No. 196 | 60 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.0167(uM) | - | Substrate: PKA-active PKA-inhibitor Products: inhibited-PKA | This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though. |