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Reaction List for pathway CaMKII (Pathway Number 245) in Accession AMPAR_traff_model1 (Accession Number 60)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name		Pathway Name / 
Pathway No.		KfKbKdtauReagents
1 CaMKII-bind-CaMCaMKII

Pathway No. 245		49.9997
(uM^-1 s^-1)		5
(s^-1)		Kd(bf) = 0.1(uM)-  Substrate:
 CaM-Ca4
 CaMKII

 Products:
 CaMKII-CaM
  This is tricky. There is some cooperativity here arising from interactions between the subunits of the CAMKII holoenzyme. However, the stoichiometry is 1. Kb/Kf = 6e4 #/cell. Rate is fast (see Hanson et al Neuron 12 943-956 1994) so lets say kb = 10. This gives kf = 1.6667e-4 H&S AnnRev Biochem 92 give tau for dissoc as 0.2 sec at low Ca, 0.4 at high. Low Ca = 100 nM = physiol.
2 
  • CaMK-thr286-bind
    -CaM
    		• CaMKII

    Pathway No. 245    		1000.19
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-  Substrate:
     CaMKII-thr286
     CaM-Ca4

     Products:
  •  CaMKII-thr286*-C
    aM
    •   Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3
    3 back_2CaMKII

    Pathway No. 245    		0.3
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-  Substrate:
  •  CaMKII-thr305-PS
    D

     Products:
     CaMK-thr305
     NMDAR
    •   Same as for back_1
    4 back_1CaMKII

    Pathway No. 245    		0.3
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-  Substrate:
     CaMKII-PSD

     Products:
     CaMKII
     NMDAR
      Rates set by the translocation experiments of Shen and Meyer, Science 1999.
    5 transloc_1CaMKII

    Pathway No. 245    		0
    (#^-1 s^-1)    		0
    (s^-1)    		Not applicable**-  Substrate:
     CaMKII-CaM
     NMDAR

     Products:
     CaMKII-CaM-PSD
      Rates to match curve in fig2 from Shen and Meyer, Science 284:162-166(1999), calculated for 6:1 alpha:beta CaMKII heterodimers
    6 transloc_2CaMKII

    Pathway No. 245    		0
    (#^-1 s^-1)    		0
    (s^-1)    		Not applicable**-  Substrate:
  •  CaMKII-thr286*-C
    aM
     NMDAR

     Products:
  •  CaMKII-thr286-Ca
    M-PSD
    •   Same as for transloc_1
    7 
  • Stoch_Basal_
    CaMKII_PSD
    		• CaMKII

    Pathway No. 245    		1
    (s^-1)    		1
    (s^-1)    		Keq = 1(uM)0.5sec  Substrate:
  •  basal_CaMKII_
    PSD_control

     Products:
  •  basal_CaMKII_
    PSD
    • 8 CaMKII-diss-CaMCaMKII

    Pathway No. 245    		5
    (s^-1)    		0
    (uM^-1 s^-1)    		--  Substrate:
     CaMKII-CaM-PSD

     Products:
     CaM-Ca4-PSD
     CaMKII-PSD
    9 
  • CaMKII-bind-CaM-
    PSD
    		• CaMKII

    Pathway No. 245    		49.9998
    (uM^-1 s^-1)    		0
    (s^-1)    		--  Substrate:
     CaMKII-PSD
     CaM-Ca4-PSD

     Products:
     CaMKII-CaM-PSD
    10 
  • CaMKII-thr286-bi
    nd-CaM-PSD
    		• CaMKII

    Pathway No. 245    		1000.02
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.0001(uM)-  Substrate:
  •  CaMKII-thr286-PS
    D
     CaM-Ca4-PSD

     Products:
  •  CaMKII-thr286-Ca
    M-PSD
    •   Same values as for the main compartment Can the main compartment pool of Ca/CaM be used?
     
    Result: 1 - 10 of 10 rows are displayed


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