DOQCS Database

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	Molecule Name/ Site Name	Km (uM)	kcat (1/s)	Ratio (k2/k3)	Enzyme Type	Substrate	Product
1	Enzyme Activity: basal_GEF_ activity Enzyme Molecule: inact-GEF	10.101	0.02	4	explicit E-S complex	GDP-Ras	GTP-Ras
2	Enzyme Activity: CaM-GEF-act-ras Enzyme Molecule: CaM-GEF	0.5051	0.2	4	explicit E-S complex	GDP-Ras	GTP-Ras
	Kinetics same as GEF-bg_act-ras
3	Enzyme Activity: GAP-inact-ras Enzyme Molecule: GAP	1.0104	10	4	explicit E-S complex	GTP-Ras	GDP-Ras
	From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
4	Enzyme Activity: GEF_ star-act-ras Enzyme Molecule: GEF_star	0.5051	0.02	4	explicit E-S complex	GDP-Ras	GTP-Ras
	Kinetics same as GEF-bg-act-ras

Enzyme List