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Enzyme List for pathway NOS (Pathway Number 90)

 Molecule Name/
Site Name 		Km (uM) kcat (1/s)Ratio
(k2/k3)		Enzyme TypeSubstrate Product
1 Enzyme Activity:
kenz

Enzyme Molecule:
Ca-CaMnNOS		1016.6674explicit E-S complexLarg
NO
cit
  Km for purified NOS is estimated between 1 - 10 uM. (Prog in Neurobiology, 2001, 64: 365-391) Vmax for unphosporylated NOS, the active form, is 500-1500 nmol/nmol/min (Montellano et al., 1998, JBC,26(12): 1185-1189). Hayashi et al., JBC, 1999, 274(29):20597-20602 report Vmax (nmol/min/mg) of nNOS Unphosporylated at 95.7 (+-) 4.2
2 Enzyme Activity:
kenz

Enzyme Molecule:
CaMKIV		5184explicit E-S complexnNOS
NOS*
  Hayashi et al., 1999, JBC,274(29):20597-20602. and other reported data from different sources.
3 Enzyme Activity:
kenz

Enzyme Molecule:
CaMKIIalpha		528.54explicit E-S complexnNOS
NOS*
  Hayashi et al., 1999, JBC,274(29):20597-20602. and from various other literature datas.
4 Enzyme Activity:
kenz

Enzyme Molecule:
CaMKIalpha		5174explicit E-S complexnNOS
NOS*
  enzyme parameters used from different literature. Hayashi et al., 1999, JBC,274(29):20597-20602.
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