| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | A1 | 0.0427 | 390 | No |
| | First adaptor protein. Tot.no. 10^4 no./cell from Asthagiri and Lauffenburger 2001 |
| 2 | A1A2 | 0 | 390 | No |
| | Adaptor protein heterodimer from Asthagiri and Lauffenburger 2001. |
| 3 | A2 | 0.0427 | 390 | No |
| | Second adaptor protein. Tot.no. 10^4 no./cell from Asthagiri and Lauffenburger 2001 |
| 4 | C | 0 | 390 | No |
| | Ligand bound receptor from Asthagiri and Lauffenburger 2001 |
| 5 | C* | 0 | 390 | No |
| | Activated form of receptor from Asthagiri and Lauffenburger 2001 |
| 6 | C*A1 | 0 | 390 | No |
| | Adaptor protein bound activated receptor from Asthagiri and Lauffenburger 2001 |
| 7 | C2 | 0 | 390 | No |
| | Dimer formed by receptor binding from Asthagiri and Lauffenburger 2001 |
| 8 | E0* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 9 | E1 | 0.0427 | 390 | No |
| | E1^T = 10^4 no./cell from Asthagiri and Lauffenburger 2001 |
| 10 | E1* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 11 | E2 | 0.0427 | 390 | No |
| | E2^T =10^4 no./cell from Asthagiri and Lauffenburger 2001. |
| 12 | E2* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 13 | E2- | 0 | 390 | No |
| | Nonfunctional second enzyme of enzyme cascade E2- from Asthagiri and Lauffnburger, 2001 |
| 14 | E3 | 0.0427 | 390 | No |
| | E3^T =10^4 no./cell from Asthagiri and Lauffenburger 2001. |
| 15 | E3* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 16 | E4 | 0.0427 | 390 | No |
| | E4^T =10^4 no./cell from Asthagiri and Lauffenburger 2001 |
| 17 | E4* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 18 | E5 | 0.0427 | 390 | No |
| | E5^T = 10^4 no./cell from Asthagiri and Lauffenburger 2001 |
| 19 | E5* | 0 | 390 | No |
| | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
| 20 | L | 0.03 | 390 | Yes |
| | L CoInit = buffered at 0.03 uM from Asthagiri, A.R and Lauffenburger, D.A Biotechnol. Prog. 2001, 17, 227-239. |
| 21 | P1 | 0.0214 | 390 | No |
| | Deactivating enzyme, P1^T = 5000 no./cell from Asthagiri and Lauffenburger, 2001 |
| 22 | P2 | 0.0214 | 390 | No |
| | Deactivating enzyme P2^T = 5000 no./cell. from Asthagiri and Lauffenburger, 2001. |
| 23 | P3 | 0.0214 | 390 | No |
| | Deactivating enzyme P3^T = 5000 no./cell from Asthagiri and Lauffenburger, 2001 |
| 24 | P4 | 0.0214 | 390 | No |
| | Deactivating enzyme P4^T = 5000 no./cell. from Asthagiri and Lauffenburger, 2001 |
| 25 | P5 | 0.0214 | 390 | No |
| | Deactivating enzyme P5^T = 5000 no./cell from Asthagiri and Lauffenburger, 2001. |
| 26 | R | 0.4274 | 390 | No |
| | R nInit = 10^5 no./cell from Asthagiri, A.R and Lauffenburger, D.A Biotechnol. Prog. 2001. 17,227-239. |
