| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | APC | 30 | 1000 | Yes |
| | arachodonylphosphatidylcholine is the favoured substrate from Wijkander and Sundler, JBC 202 pp 873-880, 1991. Their assay used 30 uM substrate, which is what the kinetics in this model are based on. For the later model we should locate a more realistic value for APC. |
| 2 | DAG-Ca-PLA2* | 0 | 1000 | No |
| | |
| 3 | PIP2-Ca-PLA2* | 0 | 1000 | No |
| | |
| 4 | PIP2-PLA2* | 0 | 1000 | No |
| | |
| 5 | PLA2* | 0 | 1000 | No |
| | |
| 6 | PLA2*-Ca | 0 | 1000 | No |
| | Phosphorylated form of PLA2. Still need to hook it up using kinases. PKA: Wightman et al JBC 257 pp6650 1982 PKC: Many refs, eg Gronich et al JBC 263 pp 16645, 1988 but see Lin etal MAPK: Lin et al, Cell 72 pp 269, 1993. Show 3x with MAPK but not PKC alone Do not know if there is a Ca requirement for active phosphorylated state. |
| 7 | PLA2-Ca* | 0 | 1000 | No |
| | |
| 8 | PLA2-cytosolic | 0.4 | 1000 | No |
| | Calculated cytosolic was 20 nm from Wijkander and Sundler However, Leslie and Channon use about 400 nM. Need to confirm, but this is the value I use here. Another recalc of W&S gives 1uM |
