| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | CaM | 26.3333 | 0.09 | No |
| | There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
| 2 | CaM-Ca3 | 0 | 0.09 | No |
| | |
| 3 | CaM-Ca3-PSD | 0 | 0.01 | No |
| | |
| 4 | CaM-Ca4 | 0 | 0.09 | No |
| | |
| 5 | CaM-Ca4-PSD | 0 | 0.01 | No |
| | |
| 6 | CaM-PSD | 26.3333 | 0.01 | No |
| | There is a LOT of this in the cell: upto 1% of total protein mass. (Alberts et al) Say 25 uM. Meyer et al Science 256 1199-1202 1992 refer to studies saying it is comparable to CaMK levels. |
| 7 | CaM-TR2-Ca2 | 0 | 0.09 | No |
| | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. |
| 8 | CaM-TR2-Ca2-PSD | 0 | 0.01 | No |
| | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. |
| 9 | neurogranin | 10 | 0.09 | No |
| | Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding .... |
| 10 | neurogranin* | 0 | 0.09 | No |
| | The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
| 11 | neurogranin*_PSD | 0 | 0.01 | No |
| | The phosph form does not bind CaM (Huang et al ABB 305:2 570-580 1993) |
| 12 | neurogranin-CaM | 0 | 0.09 | No |
| | |
| 13 | neurogranin-CaM_PSD | 0 | 0.01 | No |
| | |
| 14 | neurogranin_PSD | 10 | 0.01 | No |
| | Also known as RC3 and p17 and BICKS. Conc in brain >> 2 uM from Martzen and Slemmon J neurosci 64 92-100 1995 but others say less without any #s. Conc in dend spines is much higher than overall, so it could be anywhere from 2 uM to 50. We will estimate 10 uM as a starting point. Gerendasy et al JBC 269:35 22420-22426 1994 have a skeleton model (no numbers) indicating CaM-Ca(n) binding .... |
