|   | Name  |  Initial Conc. (uM)                   td> |  Volume (fL) |  Buffered  | 
| 1 | GDP | 0 | 12000 | No | 
|     |   GDP released from RCC1-GDP.Ran complex Nuclear Volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2  Gorlich D et al (2003)  EMBO J 22(5):1088-1100 | 
| 2 | GDP.Ran-nuclear | 0 | 12000 | No | 
|     |   nuclear-GDP.Ran  Nuclear fraction of GDP bound Ran  Approximately 71% of RanGDP in the nucleus is bound Nuclear volume = 1.2 pL = 1.2e-15 m^3 Gorlich D et al (2003) EMBO J 22(5):1088-1100 | 
| 3 | GTP | 500.007 | 12000 | No | 
|     |   GTP Initial GTP concentration = 500uM Nuclear volume = 1.2 pL = 1.2e-15 m^3 Table II footnote Gorlich D et al (2003)  EMBO J 22(5):1088-1100 | 
| 4 | GTP.Ran-nuclear | 0 | 12000 | No | 
|     |   nuclear-GTP.Ran  Nuclear fraction of Ran-GTP complex Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2  Gorlich D et al (2003)  EMBO J 22(5):1088-1100 | 
| 5 | nuclear-NTF2-GDP.Ran | 0 | 12000 | No | 
|     |   NTF2-GDP.Ran  Cytoplasmic fraction of GDP bound Ran  complexed with NTF2 Nuclear volume = 1.2 pL = 1.2e-15 m^3  Footnote for Table 2 Gorlich D et al (2003) EMBO J 22(5):1088-1100 Initial cytoplasmic GDP.Ran concentration = 5 uM  Table II footnote | 
| 6 | RCC1 | 0.7 | 12000 | No | 
|     |   RCC1  Nuclear volume = 1.2 pL = 1.2e-15 m^3 Nuclear concentration of RCC1 = 0.7 uM Supplementary Table B Gorlich D et al (2003)  EMBO J 2003, 22(5):1088-1100 | 
| 7 | RCC1-GDP.Ran | 0 | 12000 | No | 
|     |   RCC1-GDP.Ran  Ran-GDP complex bound to RCC1 Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. Nuclear volume = 1.2pL = 1.2e-15 m^3 Gorlich D et al (2003)  EMBO J 22(5):1088-1100 | 
| 8 | RCC1-Ran | 0 | 12000 | No | 
|     |   RCC1-GDP.Ran  Ran-GDP complex bound to RCC1 Nuclear volume = 1.2pL = 1.2e-15 m^3  Footnote for Table 2 Gorlich D et al (2003)  EMBO J 22(5):1088-1100 | 
| 9 | RCC1-RanGTP | 0 | 12000 | No | 
|     |   RCC1-GTP.Ran  Ran-GTP complex bound to RCC1 Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2 Gorlich D et al (2003)  EMBO J 22(5):1088-1100 |