| | Name | Initial Conc. (uM) | Volume (fL) | Buffered |
| 1 | AA | 6.12 | 1000 | No |
| | |
| 2 | Ca | 0.08 | 1000 | Yes |
| | |
| 3 | DAG | 11.661 | 1000 | Yes |
| | Baseline is 11.661 from running combined model to steady state. See ALL/NOTES for 23 Apr 1998. |
| 4 | MAPK* | 0 | 1000 | No |
| | This version is phosphorylated on both the tyr and thr residues and is active: refs The rate consts are very hard to nail down. Combine Sanghera et al JBC 265(1) :52-57 with Nemenoff et al JBC 93 pp 1960 to get k3=10/sec = k2 (from Nemenoff Vmax) and k1 = (k2 + k3)/Km = 1.3e-6 Or: k3 = 10, k2 = 40, k1 = 3.25e-6 |
| 5 | MKP-1 | 0.0027 | 1000 | No |
| | MKP-1 deph and inact MAPK in vivo: Sun et al Cell 75 487-493 1993. Kinetics from Charles et al PNAS 90:5292-5296 1993. They cite Charles et al Oncogene 7 187-190 for half-life of MKP1/3CH is 40 min. 80% deph of MAPK in 20 min Sep 17 1997: CoInit now 0.4x to 0.0032. See parm searches from jun96 on. 30 May 2k: CoInit now 0.0027, but not so critical as its prdn is regulated. |
| 6 | PKC-active | 0 | 1000 | No |
| | Conc of PKC in brain is about 1 uM (?) |
| 7 | PPhosphatase2A | 0.224 | 1000 | No |
| | Refs: Pato et al Biochem J 293:35-41(93); Takai&Mieskes Biochem J 275:233-239 k1=1.46e-4, k2=1000,k3=250. these use kcat values for calponin. Also, units of kcat may be in min! revert to Vmax base: k3=6, k2=25,k1=3.3e-6 or 6,6,1e-6 CoInit assumed 0.1 uM. See NOTES for MAPK_Ras50.g. CoInit now 0.08 Sep 17 1997: Raise CoInt 1.4x to 0.224, see parm searches from jun 96 on. |
| 8 | temp-PIP2 | 2.5 | 1000 | Yes |
| | This isn't explicitly present in the M&L model, but is obviously needed. I assume its conc is fixed at 1uM for now, which is a bit high. PLA2 is stim 7x by PIP2 @ 0.5 uM (Leslie and Channon BBA 1045:261(1990) Leslie and Channon say PIP2 is present at 0.1 - 0.2mol% range in membs, which comes to 50 nM. Ref is Majerus et al Cell 37 pp 701-703 1984 Lets use a lower level of 30 nM, same ref.... |
