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Reaction List for pathway AC (Pathway Number 221)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 CaM-bind-AC149.9998
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.02(uM)-CaM-Ca4
AC1
AC1-CaM
  Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5
2 CaM_bind_PDE1720
(uM^-1 s^-1)		5
(s^-1)		Kd(bf) = 0.0069(uM)-PDE1
CaM-Ca4
CaM.PDE1
  For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3
3 dephosph-AC20.1
(s^-1)		0
(s^-1)		--AC2*
AC2
  Random rate.
4 dephosph-PDE0.1
(s^-1)		0
(s^-1)		--cAMP-PDE*
cAMP-PDE
  The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.
5 Gs-bind-AC1126
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.0079(uM)-Gs-alpha
AC1
AC1-Gs
  Half-max 8nM from Tang et al JBC266:13 8595-8603 kb/kf = 8 nM = 4800#/cell so kf = kb * 2.08e-4
6 Gs-bind-AC2499.998
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.002(uM)-AC2
Gs-alpha
AC2-Gs
  Half-max at around 3nM = kb/kf from fig 5 in Feinstein et al PNAS USA 88 10173-10177 1991 kf = kb/1800 = 5.56e-4 kb Ofer's thesis data indicates it is more like 2 nM. kf = kb/1200 = 8.33e-4
7 Gs-bind-AC2*833.28
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.0012(uM)-Gs-alpha
AC2*
AC2*-Gs
  kb/kf = 1.2 nM so kf = kb/720 = 1.3888 * kb.
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