Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Reaction List for pathway AC (Pathway Number 241)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 CaM-bind-AC149.9997
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.02(uM)-CaM-Ca4
AC1
AC1-CaM
  Half-max at 20 nM CaM (Tang et al JBC 266:13 8595-8603 1991 kb/kf = 20 nM = 12000 #/cell so kf = kb/12000 = kb * 8.333e-5
2 cAMP_diffusion300
(s^-1)		5.4
(s^-1)		Not applicable**-cAMP
cAMP_in_dend
  Represents diffusion, from a volume of 9e-20 to 5e-18. Assuming neck dimensions of 0.1 x 0.1 microns, this works out to a diffusion const of about 270 um^2/sec, which is pretty conservative. It is what cAMP does in frog cilia.
3 CaM_bind_PDE1719.982
(uM^-1 s^-1)		5
(s^-1)		Kd(bf) = 0.0069(uM)-PDE1
CaM-Ca4
CaM.PDE1
  For olf epi PDE1, affinity is 7 nM. Assume same for brain. Reaction should be pretty fast. Assume kb = 5/sec. Then kf = 5 / (0.007 * 6e5) = 1.2e-3
4 dephosph-AC20.1
(s^-1)		0
(s^-1)		--AC2*
AC2
  Random rate.
5 dephosph-PDE0.01
(s^-1)		0
(s^-1)		--cAMP-PDE*
cAMP-PDE
  The rates for this are poorly constrained. In adipocytes (probably a different PDE) the dephosphorylation is complete within 15 min, but there are no intermediate time points so it could be much faster. Identity of phosphatase etc is still unknown.
Pathway DetailMolecule ListEnzyme ListReaction List