Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Reaction List for pathway Ras (Pathway Number 367)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 CaM-bind-GEF199.999
(uM^-1 s^-1)		1
(s^-1)		Kd(bf) = 0.005(uM)-inact-GEF
CaM-Ca4
CaM-GEF
  We have no numbers for this. It is probably between the two extremes represented by the CaMKII phosph states, and I have used guesses based on this. kf=1e-4 kb=1 The reaction is based on Farnsworth et al Nature 376 524-527 1995 28 Feb 2006: Increased affinity 36-fold to account for Ca input to MAPK cascade, possibly folding in other pathway inputs. 21 April 2006: Altered affinity to same level as pkm_mapk21.g model to prevent spontaneous turnon. Kf = 200, Kb = 1.
2 dephosph-GAP0.1
(s^-1)		0
(s^-1)		--GAP*
GAP
  Assume a reasonably good rate for dephosphorylating it, 1/sec
3 dephosph-GEF1
(s^-1)		0
(s^-1)		--GEF*
inact-GEF
4 Ras-intrinsic-GT
Pase		0.0001
(s^-1)		0
(s^-1)		--GTP-Ras
GDP-Ras
  This is extremely slow (1e-4), but it is significant as so little GAP actually gets complexed with it that the total GTP turnover rises only by 2-3 X (see Gibbs et al, JBC 265(33) 20437-20422) and Eccleston et al JBC 268(36) 27012-27019 kf = 1e-4
Pathway DetailMolecule ListEnzyme ListReaction List