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Reaction List for pathway NOS (Pathway Number 66) | | Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered. |
| | Name | Kf | Kb | Kd | tau | Substrate | Product | | 1 |
arg_bind_nNOS | 0.5
(uM^-1 s^-1) | 81
(s^-1) | Kd(bf) = 162(uM) | - | L-Arg
Ca-CaM_nNOS
| FeIII
| | | From Stuehr et al., Biochem, 1999, 38(38):12446-12451. Rates used as it is from their results. | | 2 |
Ca-CaMbind-nNOS | 3.25
(uM^-1 s^-1) | 0.05
(s^-1) | Kd(bf) = 0.0154(uM) | - | nNOS
CaM-Ca4
| Ca-CaM_nNOS
| | | Those binding CaM have a high Kd, including nNOS, ~ <= 10 nM. The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity. (Marletta, Biochemistry, 1997;36;12337-12345) | | 3 |
k1 | 10.4
(s^-1) | 0
(s^-1) | - | - | FeIII
| FeII
| | | Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243. All the rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 10.4 | | 4 |
k10 | 1.2
(uM^-1 s^-1) | 0
(uM^-1 s^-1) | - | - | FeII_NO
O2
| FeIII
NO3
| | | The rates were obtained from Stuehr et al, JBC 2001, 276(2): 1233-1243.... The rates are multiplied by 2X for every 10 C rise, to get it to the cell temp. kf -- 0.0052 | | 5 |
k2 | 3.6
(s^-1) | 0
(s^-1) | - | - | FeII
| FeII-O2
| | | Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 3.6 | | 6 |
k3 | 104
(s^-1) | 0
(s^-1) | - | - | FeII-O2
| FeIII_a
| | | Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 104 | | 7 |
k4 | 10.4
(s^-1) | 0
(s^-1) | - | - | FeIII_a
| FeII_a
| | | rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 10.4 | | 8 |
k5 | 3.6
(s^-1) | 0
(s^-1) | - | - | FeII_a
| FeII-O2_a
| | | Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243, rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 3.6 | | 9 |
k6 | 104
(s^-1) | 0
(s^-1) | - | - | FeII-O2_a
| FeIII_NO
| | | Rates from Stuehr et al., 2001, JBC, 276(2): 1233-1243. The transformation rate of FeII-O2_a to FeIII_NO was 26 /s.rates increased by 2X for every 10 C to get it to the cell temp...(thru personal correspondence) kf -- 104 | | 10 |
k9 | 0.0004
(s^-1) | 0
(s^-1) | - | - | FeII_NO
| FeII
| | | Rates from Stuehr et al., 2001, JBC 276(2): 1233-1243.., rates increased by 2X for every 10 C rise, to get it to the cell temp..(thru personal correspondence) kf -- 0.0004 | | 11 |
kF | 20
(s^-1) | 0
(s^-1) | - | - | FeIII_NO
| FeIII
| | | Rates from Stuehr et al., JBC, 2001; 276(2): 1233-1243., rates increased by 2X for every 10 C rise, to get it ot the cell temp..(thru personal correspondence) kf -- 20 | | 12 |
kG | 10.4
(s^-1) | 0
(s^-1) | - | - | FeIII_NO
| FeII_NO
| | | Rates from Stuehr et al., 2001, JBC, 276(2): 1233-1243.. rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 10.4 |
Pathway Details Molecule List Enzyme List Reaction List
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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