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Reaction List for pathway NOS (Pathway Number 66)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 arg_bind_nNOS0.5
(uM^-1 s^-1)		81
(s^-1)		Kd(bf) = 162(uM)-L-Arg
Ca-CaM_nNOS
FeIII
  From Stuehr et al., Biochem, 1999, 38(38):12446-12451. Rates used as it is from their results.
2 Ca-CaMbind-nNOS3.25
(uM^-1 s^-1)		0.05
(s^-1)		Kd(bf) = 0.0154(uM)-nNOS
CaM-Ca4
Ca-CaM_nNOS
  Those binding CaM have a high Kd, including nNOS, ~ <= 10 nM. The binding of CaM to NOS has been demonstrated to act as the trigger necessary for electron transfer and catalytic activity. (Marletta, Biochemistry, 1997;36;12337-12345)
3 k110.4
(s^-1)		0
(s^-1)		--FeIII
FeII
  Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243. All the rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 10.4
4 k101.2
(uM^-1 s^-1)		0
(uM^-1 s^-1)		--FeII_NO
O2
FeIII
NO3
  The rates were obtained from Stuehr et al, JBC 2001, 276(2): 1233-1243.... The rates are multiplied by 2X for every 10 C rise, to get it to the cell temp. kf -- 0.0052
5 k23.6
(s^-1)		0
(s^-1)		--FeII
FeII-O2
  Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 3.6
6 k3104
(s^-1)		0
(s^-1)		--FeII-O2
FeIII_a
  Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 104
7 k410.4
(s^-1)		0
(s^-1)		--FeIII_a
FeII_a
  rates from Stuehr et al., 2001, JBC, 276(2):1233-1243 rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 10.4
8 k53.6
(s^-1)		0
(s^-1)		--FeII_a
FeII-O2_a
  Rates from Stuehr et al., 2001, JBC, 276(2):1233-1243, rates increased by 2X for every 10 C rise to get it to the cell temp.. (thru personal correspondence) kf -- 3.6
9 k6104
(s^-1)		0
(s^-1)		--FeII-O2_a
FeIII_NO
  Rates from Stuehr et al., 2001, JBC, 276(2): 1233-1243. The transformation rate of FeII-O2_a to FeIII_NO was 26 /s.rates increased by 2X for every 10 C to get it to the cell temp...(thru personal correspondence) kf -- 104
10 k90.0004
(s^-1)		0
(s^-1)		--FeII_NO
FeII
  Rates from Stuehr et al., 2001, JBC 276(2): 1233-1243.., rates increased by 2X for every 10 C rise, to get it to the cell temp..(thru personal correspondence) kf -- 0.0004
11 kF20
(s^-1)		0
(s^-1)		--FeIII_NO
FeIII
  Rates from Stuehr et al., JBC, 2001; 276(2): 1233-1243., rates increased by 2X for every 10 C rise, to get it ot the cell temp..(thru personal correspondence) kf -- 20
12 kG10.4
(s^-1)		0
(s^-1)		--FeIII_NO
FeII_NO
  Rates from Stuehr et al., 2001, JBC, 276(2): 1233-1243.. rates increased by 2X for every 10 C rise, to get it to the cell temp.. (thru personal correspondence) kf -- 10.4
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