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Enzyme List for pathway AC (Pathway Number 136) in Accession cAMP_pathway (Accession Number 25)

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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 cAMP-PDE /
PDE
AC

Pathway No. 136
19.8413104explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  Best rates are from Conti et al Biochem 34 7979-7987 1995. Though these are for the Sertoli cell form, it looks like they carry nicely into alternatively spliced brain form. See Sette et al JBC 269:28 18271-18274 Km ~2 uM, Vmax est ~ 10 umol/min/mg for pure form. Brain protein is 93 kD but this was 67. So k3 ~10, k2 ~40, k1 ~4.2e-6
2 cAMP-PDE* /
PDE*
AC

Pathway No. 136
19.8413204explicit E-S complexSubstrate: 
cAMP
Product : 
AMP
  This form has about twice the activity of the unphosphorylated form. See Sette et al JBC 269:28 18271-18274 1994. We'll ignore cGMP effects for now.
3 Gs.AC /
cyclase
AC

Pathway No. 136
20184Classical Michaelis-Menten
V = Etot.S.Kcat/Km+S
Substrate: 
ATP
Product : 
cAMP
  See Feinstein et al PNAS 88:10173-10177, Jacobowitz et al JBC 286(6):3829-3832 Smigel, JBC 261(4):1976-1982 (1986)

 
Result: 1 - 3 of 3 rows are displayed



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