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Result: 1 - 5 of 5 rows are displayed

Enzyme List for pathway PLA2 (Pathway Number 945) in Accession Ajay_Bhalla_2007_ReacDiff3 (Accession Number 84)

Entries are grouped according to Pathway Number and they are alternately color coded using  and   color.
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  Enzyme Molecule /

 Enzyme Activity
Pathway Name / 
Pathway No.
Km
(uM)
kcat
(s^-1)
Ratio
(k2/k3)
Enzyme TypeReagents
1 PLA2-Ca* /
kenz
PLA2

Pathway No. 945
19.99945.44explicit E-S complexSubstrate: 
APC
Product : 
AA
  10 x raise oct22 12 x oct 24, set k2 = 4 * k3
2 PIP2-PLA2* /
kenz
PLA2

Pathway No. 945
19.999811.044explicit E-S complexSubstrate: 
APC
Product : 
AA
  10 X raise oct 22 12 X further raise oct 24 to allow for correct conc of enzyme
3 PIP2-Ca-PLA2* /
kenz
PLA2

Pathway No. 945
20364explicit E-S complexSubstrate: 
APC
Product : 
AA
  10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.
4 DAG-Ca-PLA2* /
kenz
PLA2

Pathway No. 945
20604explicit E-S complexSubstrate: 
APC
Product : 
AA
  10 X raise oct 22 12 X raise oct 24 + conversion to k2 =4 * k3
5 PLA2*-Ca /
kenz
PLA2

Pathway No. 945
201204explicit E-S complexSubstrate: 
APC
Product : 
AA
  This form should be 3 to 6 times as fast as the Ca-only form. I have scaled by 4x which seems to give a 5x rise. 10x raise Oct 22 12 x oct 24, changed k2 = 4 * k3

 
Result: 1 - 5 of 5 rows are displayed



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