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Result: 1 - 5 of 5 rows are displayed

Reaction List for pathway CaM (Pathway Number 259) in Accession CaMKII_noPKA_model3 (Accession Number 62)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 CaM-TR2-bind-CaCaM

Pathway No. 259
71.999
(uM^-2 s^-1)
72
(s^-1)
Kd(af) = 1(uM)-  Substrate:
 CaM
 Ca
 Ca

 Products:
 CaM-TR2-Ca2
  Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !)....
2 
  • CaM-TR2-Ca2-bind
    -Ca
  • CaM

    Pathway No. 259
    3.6
    (uM^-1 s^-1)
    10
    (s^-1)
    Kd(bf) = 2.7778(uM)-  Substrate:
     CaM-TR2-Ca2
     Ca

     Products:
     CaM-Ca3
      K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10
    3 
  • CaM-TR2-bind-Ca-
    PSD
  • CaM

    Pathway No. 259
    72
    (uM^-2 s^-1)
    72
    (s^-1)
    Kd(af) = 1(uM)-  Substrate:
     CaM-PSD
     Ca-PSD
     Ca-PSD

     Products:
     CaM-TR2-Ca2-PSD
      Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !)....
    4 
  • CaM-TR2-Ca2-bind
    -Ca-PSD
  • CaM

    Pathway No. 259
    3.6
    (uM^-1 s^-1)
    10
    (s^-1)
    Kd(bf) = 2.7778(uM)-  Substrate:
     CaM-TR2-Ca2-PSD
     Ca-PSD

     Products:
     CaM-Ca3-PSD
      K3 = 21.5, K4 = 2.8. Assuming that the K4 step happens first, we get kb/kf = 2.8 uM = 1.68e6 so kf =6e-6 assuming kb = 10
    5 
  • CaM-Ca3-bind-Ca-
    PSD
  • CaM

    Pathway No. 259
    0.465
    (uM^-1 s^-1)
    10
    (s^-1)
    Kd(bf) = 21.5048(uM)-  Substrate:
     CaM-Ca3-PSD
     Ca-PSD

     Products:
     CaM-Ca4-PSD
      Use K3 = 21.5 uM here from Stemmer and Klee table 3. kb/kf =21.5 * 6e5 so kf = 7.75e-7, kb = 10

     
    Result: 1 - 5 of 5 rows are displayed



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