NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By


 
Result: 1 - 3 of 3 rows are displayed

Reaction List for pathway PLC_g (Pathway Number 79) in Accession Synaptic_Network (Accession Number 16)

Entries are grouped according to Pathway Number and they are alternately color coded using  and  color.
Further ordering can be done to the table header.  indicates that ordering is done according to ascending or descending order.
Keq is calculated only for first order reactions.
Kd is calculated only for second order reactions. [nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules]
  Reaction
Name
Pathway Name / 
Pathway No.
KfKbKdtauReagents
1 Ca_act_PLC_gPLC_g

Pathway No. 79
180
(uM^-1 s^-1)
10
(s^-1)
Kd(bf) = 0.0556(uM)-  Substrate:
 PLC_g
 Ca

 Products:
 Ca.PLC_g
  Nice curves from Homma et al JBC 263:14 6592-6598 1988 Fig 5c. The activity falls above 10 uM, but that is too high to reach physiologically anyway, so we'll ignore the higher pts and match the lower ones only. Half-max at 1 uM. But Wahl et al JBC 267:15 10447-10456 1992 have half-max at 56 nM which is what I'll use.
2 Ca_act_PLC_g*PLC_g

Pathway No. 79
12
(uM^-1 s^-1)
10
(s^-1)
Kd(bf) = 0.8333(uM)-  Substrate:
 Ca
 PLC_G*

 Products:
 Ca.PLC_g*
  Again, we refer to Homma et al and Wahl et al, for preference using Wahl et al JBC 267(15):10447-10456 1992. Half-Max of the phosph form is at 316 nM. Use kb of 10 as this is likely to be pretty fast. As we are phosphorylating the Ca-bound form, equils have shifted. kf should now be 2e-5 (Kf = 12) to match the reported half-max.
3 dephosph_PLC_gPLC_g

Pathway No. 79
0.05
(s^-1)
0
(s^-1)
--  Substrate:
 Ca.PLC_g*

 Products:
 Ca.PLC_g
  This is a generic balancing dephosphorylation step for the PLC. Rates are determined by considering balance between phosph and non-phosph forms of PLC-gamma. Wahl et al JBC 267(15) 10447-10456 1992 put the ratio at 50% phosph form in cytoplasm, about 20% in membrane.

 
Result: 1 - 3 of 3 rows are displayed



Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.