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Molecule Parameter List for PLC

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PLC participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1010020

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • NonOsc_Ca_
    IP3metabolism
  • 31Network
    MIPP CaMKII CaM 
    PKC IP3-3K CaRegulation 
    Gq PLCbeta 134_dephos 
    145_dephos IP4-system IHP-system 
    1345_dephos 
    This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

    PLC acting as a Molecule in  
    NonOsc_Ca_IP3metabolism Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    PLC
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PLCbeta
    Pathway No. : 151
    0.041000No
    Smrcka et al; Science 251, 15.2.1991, pp804-807

    PLC acting as an Enzyme in  
    NonOsc_Ca_IP3metabolism Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PLC /
    PLC
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PLCbeta
    Pathway No. : 151
    19.99942.54explicit E-S complexSubstrate
    PIP2

    Product
    DAG
    IP3(145)
    Smrcka et al; Science 251, 15.2.1991, pp804-807

    PLC acting as a Substrate in a reaction in  
    NonOsc_Ca_IP3metabolism Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
     NameAccession NamePathway NameKfKbKdtauReagents
    1Act-PLC-Ca
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PLCbeta
    Pathway No. : 151
    3
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.3333(uM)-Substrate
    Ca
    PLC

    Product
    PLC-Ca
      Affinity for Ca = 1uM without AlF, 0.1 with: from Smrcka et al science 251 pp 804-807 1991 so [Ca].kf = kb so kb/kf = 1 * 6e5 = 1/1.66e-6 Assigned affinity to a Kd of 0.333 to maintain balance.
    2PLC-bind-Gq
  • NonOsc_Ca_
    IP3metabolism

    Accession No. : 31
  • PLCbeta
    Pathway No. : 151
    2.52
    (uM^-1 s^-1)
    1
    (s^-1)
    Kd(bf) = 0.3968(uM)-Substrate
    G*GTP
    PLC

    Product
    PLC-Gq
      this binding does not produce active PLC. This step was needed to implement the described (Smrcka et al) increase in affinity for Ca by PLC once Gq was bound. The tempkin are the same as the binding step for Ca-PLC to Gq. Kd is constrained by detailed balance.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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