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Molecule Parameter List for CaMKII-thr286

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
CaMKII-thr286 participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1103111

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
  • Ajay_Bhalla_
    2004_PKM_Tuning
  • 76Network
    PKC Shared_Object_Ajay_Bhalla_2004_PKM_tuning PLA2 
    PLCbeta Gq MAPK 
    Ras EGFR Sos 
    PLC_g CaMKII CaM 
    PP1 PP2B PKA 
    AC PKM 
    This model is taken from the Ajay SM, Bhalla US. Eur J Neurosci. 2004 Nov;20(10):2671-80. This is the reference feedforward model from Figure 8a.

    CaMKII-thr286 acting as a Molecule in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    NameAccession NamePathway NameInitial Conc.
    (uM)
    Volume
    (fL)
    Buffered
    CaMKII-thr286
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    01.5No
    I am not sure if we need to endow this one with a lot of enzs. It is likely to be a short-lived intermediate, since it will be phosphorylated further as soon as the CAM falls off.

    CaMKII-thr286 acting as a Summed Molecule in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Accession NamePathway NameTargetInput
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    tot_autonomous_CaMKIICaMKII-thr286
    CaMKII***

    CaMKII-thr286 acting as a Substrate for an Enzyme in  
    Ajay_Bhalla_2004_PKM_Tuning Network
     Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    1tot_CaM_CaMKII  /
    CaM_act_305
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    113.59964explicit E-S complexSubstrate
    CaMKII-thr286

    Product
    CaMKII***
        Rates from autocamtide phosphorylation, from Hanson and Schulman JBC 267:24 17216-17224 1992. Jan 1 1998: Speed up 12x to match fig 5.
    2
  • tot_autonomous_
    CaMKII
      /
    auton_305
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    175.00664explicit E-S complexSubstrate
    CaMKII-thr286

    Product
    CaMKII***
        See Hanson and Schulman again, for afterburst rates of phosph.
    3PP1-active  /
    Deph_thr286b
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Shared_Object_
    Ajay_Bhalla_
    2004_PKM_tuning

    Pathway No. : 312
  • 5.099110.354explicit E-S complexSubstrate
    CaMKII-thr286

    Product
    CaMKII
       

    CaMKII-thr286 acting as a Product of an Enzyme in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Enzyme Molecule /
    Enzyme Activity
    Accession NamePathway NameKm (uM)kcat (s^-1)RatioEnzyme TypeReagents
    PP1-active  /
    Deph-thr305
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • Shared_Object_
    Ajay_Bhalla_
    2004_PKM_tuning

    Pathway No. : 312
  • 5.099110.354explicit E-S complexSubstrate
    CaMKII***

    Product
    CaMKII-thr286

    CaMKII-thr286 acting as a Substrate in a reaction in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
  • CaMK-thr286-bind
    -CaM
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    1000.17
    (uM^-1 s^-1)
    0.1
    (s^-1)
    Kd(bf) = 0.0001(uM)-Substrate
    CaM-Ca4
    CaMKII-thr286

    Product
  • CaMKII-thr286*-C
    aM

  • Affinity is up 1000X. Time to release is about 20 sec, so the kb is OK at 0.1 This makes Kf around 1.6666e-3

    CaMKII-thr286 acting as a Product in a reaction in  
    Ajay_Bhalla_2004_PKM_Tuning Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    basal-activity
  • Ajay_Bhalla_
    2004_PKM_Tuning

    Accession No. : 76
  • CaMKII
    Pathway No. : 322
    0.003
    (s^-1)
    0
    (s^-1)
    --Substrate
    CaMKII

    Product
    CaMKII-thr286
    This reaction represents one of the big unknowns in CaMK-II biochemistry: what maintains the basal level of phosphorylation on thr 286 ? See Hanson and Schulman Ann Rev Biochem 1992 61:559-601, specially pg 580, for review. I have not been able to find any compelling mechanism in the literature, but fortunately the level of basal activity is well documented.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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