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Molecule Parameter List for PIP2-Ca-PLA2*

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

PIP2-Ca-PLA2* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	0	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
3d_fold_model	8	Network	Shared_Object_3d_fold_model, PKC, MAPK, PLA2, Ras
This model is an annotated version of the synaptic signaling network. The primary reference is Bhalla US and Iyengar R. Science (1999) 283(5400):381-7 but several of the model pathways have been updated Bhalla US, Ram PT, Iyengar R. Science. 2002 Aug 9;297(5583):1018-23.

PIP2-Ca-PLA2* acting as a Molecule in 3d_fold_model Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PIP2-Ca-PLA2*	3d_fold_model Accession No. : 8	PLA2 Pathway No. : 57	0	1000	No

PIP2-Ca-PLA2* acting as an Enzyme in 3d_fold_model Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PIP2-Ca-PLA2* / kenz	3d_fold_model Accession No. : 8	PLA2 Pathway No. : 57	20	36	4	explicit E-S complex	Substrate APC Product AA
10 x raise oct 22 12 x and rescale for k2 = 4 * k3 convention oct 24 Increase further to get the match to expt, which was spoilt due to large accumulation of PLA2 in the enzyme complexed forms. Lets raise k3, leaving the others at k1 = 1.5e-5 and k2 = 144 since they are large already.

PIP2-Ca-PLA2* acting as a Product in a reaction in 3d_fold_model Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
PIP2-Ca-PLA2-act	3d_fold_model Accession No. : 8	PLA2 Pathway No. : 57	0.012 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 8.3333(uM)	-	Substrate PLA2-Ca* temp-PIP2 Product PIP2-Ca-PLA2*

Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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