|
Enter a Search String | | Special character and space not allowed in the query term.
Search string should be at least 2 characters long. |
Molecule Parameter List for CaM-Ca | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Ajay_Bhalla_
2007_ReacDiff2 | 83 | Network | Shared_Object_Ajay_Bhalla_2007_ReacDiff, PKC, MAPK,
Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1],
PKC, MAPK, Ras, kinetics[3], CaM, PKM, kinetics[2], PKC, MAPK, Ras,
CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM,
PKM, kinetics[5], PKC, MAPK, Ras, CaM, PKM, kinetics[6], PKC, MAPK,
Ras, CaM, PKM, kinetics[7], PKC, MAPK, Ras, CaM, PKM, kinetics[8],
PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10],
PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12],
PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14],
PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16],
PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18],
PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20],
PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22],
PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM, kinetics[24],
PKC, MAPK, Ras, CaM, PKM, kinetics[25], PKC, MAPK, Ras, CaM, PKM, kinetics[26],
PKC, MAPK, Ras, CaM, PKM, kinetics[27], PKC, MAPK, Ras, CaM, PKM, kinetics[28],
PKC, MAPK, Ras, CaM, PKM, kinetics[29], PKC, MAPK, Ras, CaM, PKM, kinetics[30],
PKC, MAPK, Ras, CaM, PKM, kinetics[31], PKC, MAPK, Ras, CaM, PKM, kinetics[32],
PKC, MAPK, Ras, CaM, PKM, kinetics[33], PKC, MAPK, Ras, CaM, PKM, kinetics[34],
PKC, MAPK, Ras, CaM, PKM, kinetics[35], PKC, MAPK, Ras, CaM, PKM, kinetics[36],
PKC, MAPK, Ras, CaM, PKM, kinetics[37], PKC, MAPK, Ras, CaM, PKM, kinetics[38],
PKC, MAPK, Ras, CaM, PKM | This is a 40-compartment reaction-diffusion-transport version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 40 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec In addition, we have a forward (dendrite to soma) transport term of 1 microns/sec. This converts to a rate of 0.1/sec, but applies only to the kf. So the total kf of the diffusion 'reaction' is 0.11 for D = 1 micron^2/sec, and kb is 0.01. If D=0.1 micron^2/sec then kf = 0.101 and kb = 0.001. In addition this model has all molecules buffered in the first and last compartments. This boundary conditions says that the molecules are not drained out of the first compartment, nor do they all pile up in the last one.
The stimulus file pkm_mapk22_transp_endbuf_D1e-13_Fig4CD which was used for the model to replicate Figure 4C and 4D from the paper.
|
CaM-Ca acting as a Molecule in Ajay_Bhalla_2007_ReacDiff2 Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 688 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 695 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 701 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 706 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 712 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 718 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 724 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 730 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 736 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 742 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 748 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 754 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 760 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 766 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 772 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 778 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 784 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 790 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 796 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 802 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 808 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 814 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 820 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 826 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 832 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 838 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 844 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 850 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 856 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 862 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 868 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 874 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 880 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 886 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 892 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 898 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 904 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 910 | 1.472 | 1.5 | No | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 681 | 1.472 | 1.5 | Yes | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. | | CaM-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 916 | 1.472 | 1.5 | Yes | | This is the intermediate where the TR2 end (the high-affinity end) has bound the Ca but the TR1 end has not. |
CaM-Ca acting as a Substrate in a reaction in Ajay_Bhalla_2007_ReacDiff2 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 681 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 2 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 3 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 688 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 4 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 5 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 695 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 6 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 7 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 701 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 8 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 9 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 706 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 10 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 11 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 712 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 12 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 13 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 718 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 14 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 15 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 724 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 16 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 17 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 730 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 18 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 19 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 736 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 20 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 21 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 742 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 22 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 23 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 748 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 24 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 25 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 754 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 26 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 27 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 760 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 28 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 29 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 766 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 30 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 31 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 772 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 32 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 33 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 778 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 34 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 35 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 784 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 36 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 37 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 790 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 38 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 39 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 796 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 40 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 41 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 802 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 42 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 43 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 808 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 44 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 45 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 814 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 46 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 47 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 820 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 48 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 49 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 826 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 50 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 51 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 832 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 52 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 53 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 838 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 54 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 55 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 844 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 56 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 57 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 850 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 58 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 59 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 856 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 60 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 61 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 862 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 62 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 63 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 868 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 64 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 65 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 874 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 66 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 67 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 880 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 68 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 69 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 886 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 70 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 71 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 892 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 72 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 73 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 898 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 74 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 75 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 904 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 76 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 77 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 910 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 78 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 79 | CaM-Ca-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 916 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM-Ca
Product
CaM-Ca2
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 |
CaM-Ca acting as a Product in a reaction in Ajay_Bhalla_2007_ReacDiff2 Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 681 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 2 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 3 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 688 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 4 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 5 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 695 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 6 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 7 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 701 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 8 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 9 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 706 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 10 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 11 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 712 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 12 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 13 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 718 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 14 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 15 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 724 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 16 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 17 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 730 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 18 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 19 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 736 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 20 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 21 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 742 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 22 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 23 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 748 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 24 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 25 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 754 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 26 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 27 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 760 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 28 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 29 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 766 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 30 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 31 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 772 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 32 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 33 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 778 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 34 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 35 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 784 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 36 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 37 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 790 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 38 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 39 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 796 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 40 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 41 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 802 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 42 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 43 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 808 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 44 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 45 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 814 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 46 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 47 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 820 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 48 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 49 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 826 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 50 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 51 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 832 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 52 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 53 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 838 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 54 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 55 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 844 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 56 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 57 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 850 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 58 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 59 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 856 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 60 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 61 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 862 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 62 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 63 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 868 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 64 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 65 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 874 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 66 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 67 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 880 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 68 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 69 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 886 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 70 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 71 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 892 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 72 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 73 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 898 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 74 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 75 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 904 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 76 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 77 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 910 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 | | 78 | diff | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | Shared_Object_
Ajay_Bhalla_
2007_ReacDiff
Pathway No. : 677 | 0.101
(s^-1) | 0.001
(s^-1) | Keq = 0.0099(uM) | 9.804sec | Substrate
CaM-Ca
Product
CaM-Ca
| | 79 | CaM-bind-Ca | Ajay_Bhalla_
2007_ReacDiff2
Accession No. : 83 | CaM
Pathway No. : 916 | 8.4851
(uM^-1 s^-1) | 8.4853
(s^-1) | Kd(bf) = 1(uM) | - | Substrate
Ca
CaM
Product
CaM-Ca
| | | Lets use the fast rate consts here. Since the rates are so different, I am not sure whether the order is relevant. These correspond to the TR2C fragment. We use the Martin et al rates here, plus the Drabicowski binding consts. All are scaled by 3X to cell temp. kf = 2e-10 kb = 72 Stemmer & Klee: K1=.9, K2=1.1. Assume 1.0uM for both. kb/kf=3.6e11. If kb=72, kf = 2e-10 (Exactly the same !) 19 May 2006. Splitting the old CaM-TR2-bind-Ca reaction into two steps, each binding 1 Ca. This improves numerical stability and is conceptually better too. Overall rates are the same, so each kf and kb is the square root of the earlier ones. So kf = 1.125e-4, kb = 8.4853 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
|
