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Molecule Parameter List for PKC-active

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by

color.

Statistics

*PKC-active* participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	40	38	120	0	0	0	0

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
Ajay_Bhalla_ 2007_ReacDiff2	83	Network	Shared_Object_Ajay_Bhalla_2007_ReacDiff, PKC, MAPK, Ras, CaM, PKM, chain, kinetics, PKC, MAPK, Ras, CaM, PKM, kinetics[1], PKC, MAPK, Ras, kinetics[3], CaM, PKM, kinetics[2], PKC, MAPK, Ras, CaM, PKM, PKC, MAPK, Ras, CaM, PKM, kinetics[4], PKC, MAPK, Ras, CaM, PKM, kinetics[5], PKC, MAPK, Ras, CaM, PKM, kinetics[6], PKC, MAPK, Ras, CaM, PKM, kinetics[7], PKC, MAPK, Ras, CaM, PKM, kinetics[8], PKC, MAPK, Ras, CaM, PKM, kinetics[9], PKC, MAPK, Ras, CaM, PKM, kinetics[10], PKC, MAPK, Ras, CaM, PKM, kinetics[11], PKC, MAPK, Ras, CaM, PKM, kinetics[12], PKC, MAPK, Ras, CaM, PKM, kinetics[13], PKC, MAPK, Ras, CaM, PKM, kinetics[14], PKC, MAPK, Ras, CaM, PKM, kinetics[15], PKC, MAPK, Ras, CaM, PKM, kinetics[16], PKC, MAPK, Ras, CaM, PKM, kinetics[17], PKC, MAPK, Ras, CaM, PKM, kinetics[18], PKC, MAPK, Ras, CaM, PKM, kinetics[19], PKC, MAPK, Ras, CaM, PKM, kinetics[20], PKC, MAPK, Ras, CaM, PKM, kinetics[21], PKC, MAPK, Ras, CaM, PKM, kinetics[22], PKC, MAPK, Ras, CaM, PKM, kinetics[23], PKC, MAPK, Ras, CaM, PKM, kinetics[24], PKC, MAPK, Ras, CaM, PKM, kinetics[25], PKC, MAPK, Ras, CaM, PKM, kinetics[26], PKC, MAPK, Ras, CaM, PKM, kinetics[27], PKC, MAPK, Ras, CaM, PKM, kinetics[28], PKC, MAPK, Ras, CaM, PKM, kinetics[29], PKC, MAPK, Ras, CaM, PKM, kinetics[30], PKC, MAPK, Ras, CaM, PKM, kinetics[31], PKC, MAPK, Ras, CaM, PKM, kinetics[32], PKC, MAPK, Ras, CaM, PKM, kinetics[33], PKC, MAPK, Ras, CaM, PKM, kinetics[34], PKC, MAPK, Ras, CaM, PKM, kinetics[35], PKC, MAPK, Ras, CaM, PKM, kinetics[36], PKC, MAPK, Ras, CaM, PKM, kinetics[37], PKC, MAPK, Ras, CaM, PKM, kinetics[38], PKC, MAPK, Ras, CaM, PKM
This is a 40-compartment reaction-diffusion-transport version of the Ajay_Bhalla_2007_PKM model. The original single-compartment model is repeated 40 times. In addition, a subset (27 out of 42) molecules can diffuse between compartments. Diffusion is implemented as a reaction between corresponding molecules in neighboring compartments. For D = 1e-12 m^2/sec (i.e., 1 micron^2/sec ) the kf and kb of this reaction for these 10 micron compartments are both 0.01/sec In addition, we have a forward (dendrite to soma) transport term of 1 microns/sec. This converts to a rate of 0.1/sec, but applies only to the kf. So the total kf of the diffusion 'reaction' is 0.11 for D = 1 micron^2/sec, and kb is 0.01. If D=0.1 micron^2/sec then kf = 0.101 and kb = 0.001. In addition this model has all molecules buffered in the first and last compartments. This boundary conditions says that the molecules are not drained out of the first compartment, nor do they all pile up in the last one. The stimulus file pkm_mapk22_transp_endbuf_D1e-13_Fig4CD which was used for the model to replicate Figure 4C and 4D from the paper.

PKC-active acting as a Molecule in Ajay_Bhalla_2007_ReacDiff2 Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	0.0476	1.5	Yes
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	0.0476	1.5	Yes
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	0.0476	1.5	No
PKC-active	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	0.0476	1.5	No

PKC-active acting as a Summed Molecule in Ajay_Bhalla_2007_ReacDiff2 Network

	Accession Nane	Pathway Name	Target	Input
1	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

2	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

3	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

4	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

5	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

6	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

7	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

8	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

9	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

10	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

11	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

12	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

13	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

14	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

15	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

16	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

17	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

18	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

19	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

20	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

21	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

22	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

23	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

24	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

25	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

26	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

27	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

28	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

29	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

30	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

31	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

32	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

33	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

34	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

35	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

36	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

37	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

38	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	PKC-active	PKC-Ca-memb* PKC-DAG-memb* PKM-zeta

PKC-active acting as an Enzyme in Ajay_Bhalla_2007_ReacDiff2 Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
2	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
3	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	Shared_Object_ Ajay_Bhalla_ 2007_ReacDiff Pathway No. : 677	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
4	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
5	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
6	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics Pathway No. : 684	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
7	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
8	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
9	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[1] Pathway No. : 690	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
10	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
11	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
12	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[2] Pathway No. : 697	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
13	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
14	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
15	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[3] Pathway No. : 694	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
16	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
17	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
18	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[4] Pathway No. : 708	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
19	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
20	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
21	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[5] Pathway No. : 714	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
22	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
23	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
24	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[6] Pathway No. : 720	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
25	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
26	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
27	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[7] Pathway No. : 726	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
28	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
29	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
30	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[8] Pathway No. : 732	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
31	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
32	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
33	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[9] Pathway No. : 738	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
34	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
35	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
36	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[10] Pathway No. : 744	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
37	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
38	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
39	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[11] Pathway No. : 750	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
40	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
41	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
42	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[12] Pathway No. : 756	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
43	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
44	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
45	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[13] Pathway No. : 762	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
46	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
47	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
48	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[14] Pathway No. : 768	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
49	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
50	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
51	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
52	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
53	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[15] Pathway No. : 774	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
54	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
55	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
56	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[16] Pathway No. : 780	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
57	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
58	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
59	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[17] Pathway No. : 786	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
60	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
61	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
62	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[18] Pathway No. : 792	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
63	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
64	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
65	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[19] Pathway No. : 798	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
66	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
67	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
68	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[20] Pathway No. : 804	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
69	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
70	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
71	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[21] Pathway No. : 810	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
72	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
73	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
74	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[22] Pathway No. : 816	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
75	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
76	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
77	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[23] Pathway No. : 822	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
78	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
79	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
80	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[24] Pathway No. : 828	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
81	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
82	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
83	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[25] Pathway No. : 834	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
84	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
85	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
86	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[26] Pathway No. : 840	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
87	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
88	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
89	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[27] Pathway No. : 846	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
90	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
91	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
92	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[28] Pathway No. : 852	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
93	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
94	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
95	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[29] Pathway No. : 858	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
96	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
97	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
98	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[30] Pathway No. : 864	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
99	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[31] Pathway No. : 870	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
100	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
101	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
102	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[32] Pathway No. : 876	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
103	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
104	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
105	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[33] Pathway No. : 882	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
106	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
107	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
108	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[34] Pathway No. : 888	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
109	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
110	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
111	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[35] Pathway No. : 894	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
112	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
113	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
114	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[36] Pathway No. : 900	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
115	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
116	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
117	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[37] Pathway No. : 906	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X
118	PKC-active / PKC-act-raf	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	20.0005	4	4	explicit E-S complex	Substrate craf-1 Product craf-1*
	Rate consts from Chen et al Biochem 32, 1032 (1993) k3 = k2 = 4 k1 = 9e-5 recalculated gives 1.666e-5, which is not very different. Looks like k3 is rate-limiting in this case: there is a huge amount of craf locked up in the enz complex. Let us assume a 10x higher Km, ie, lower affinity. k1 drops by 10x. Also changed k2 to 4x k3. Lowerd k1 to 1e-6 to balance 10X DAG sensitivity of PKC
119	PKC-active / PKC-inact-GAP	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	3.33333	4	4	explicit E-S complex	Substrate GAP Product GAP*
	Rate consts copied from PCK-act-raf This reaction inactivates GAP. The idea is from the Boguski and McCormick review.
120	PKC-active / PKC-act-GEF	Ajay_Bhalla_ 2007_ReacDiff2 Accession No. : 83	kinetics[38] Pathway No. : 912	3.33333	4	4	explicit E-S complex	Substrate inact-GEF Product GEF*
	Rate consts from PKC-act-raf. This reaction activates GEF. It can lead to at least 2X stim of ras, and a 2X stim of MAPK over and above that obtained via direct phosph of c-raf. Note that it is a push-pull reaction, and there is also a contribution through the phosphorylation and inactivation of GAPs. The original PKC-act-raf rate consts are too fast. We lower K1 by 10 X

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