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Molecule Parameter List for PKC-DAG-AA

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
PKC-DAG-AA participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000011

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
PKC_turnover26Pathway
PKC 
This model is a superset of the PKC model presented in Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. The current version includes PKC turnover. This version includes the stimulating pools Ca, AA and DAG as well as the PKC activity within the synaptic signaling pathway since it is meant to be a self-contained pathway model rather than part of a network.

PKC-DAG-AA acting as a Molecule in  
PKC_turnover Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
PKC-DAG-AAPKC_turnover
Accession No. : 26
PKC
Pathway No. : 138
01000No
Complex of PKC, DAG and AA giving rise to synergistic activation of PKC by DAG and AA at resting Ca.

PKC-DAG-AA acting as a Substrate in a reaction in  
PKC_turnover Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
  • PKC-act-by-DAG-A
    A
  • PKC_turnover
    Accession No. : 26
    PKC
    Pathway No. : 138
    2
    (s^-1)
    0.2
    (s^-1)
    Keq = 0.1(uM)0.455secSubstrate
    PKC-DAG-AA

    Product
    PKC-DAG-AA*
    Membrane translocation step for PKC-DAG-AA complex. Rates from matching concentration-effect data in our two main references: Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and Shinomura et al 1988 PNAS 88: 5149-5153

    PKC-DAG-AA acting as a Product in a reaction in  
    PKC_turnover Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    PKC-n-DAG-AAPKC_turnover
    Accession No. : 26
    PKC
    Pathway No. : 138
    0.018
    (uM^-1 s^-1)
    2
    (s^-1)
    Kd(bf) = 111.1111(uM)-Substrate
    AA
    PKC-DAG

    Product
    PKC-DAG-AA
    This is one of the more interesting steps. Mechanisticmpdmyy it does not seem necessary at first glance. Turns out that one needs this step to quantitatively match the curves in Schaechter and Benowitz 1993 J Neurosci 13(10):4361 and Shinomura et al 1991 PNAS 88:5149-5153. There is a synergy between DAG and AA activation even at low Ca levels, which is most simply represented by this reaction. Tau is assumed to be fast. Kd comes from matching the experimental curves.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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