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Molecule Parameter List for inactiveRas-MKKK

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
inactiveRas-MKKK participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000001

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
MAPK_osc27Pathway
MAPK 
This MAPK model is based on Boris N. Kholodenko Eur J Biochem. (2000) 267(6):1583-8 for data from Xenopus oocytes extracts.

inactiveRas-MKKK acting as a Molecule in  
MAPK_osc Network
NameAccession NamePathway NameInitial Conc.
(uM)
Volume
(fL)
Buffered
inactiveRas-MKKKMAPK_osc
Accession No. : 27
MAPK
Pathway No. : 139
00.0016667No
This is the inactive form of Ras-MKKK. Based on the reaction scheme from Kholodenko 2000, this is equivalent to a binding of the MAPK-PP to the Ras. The amount of Ras in the model is small enough that negligible amounts of MAPK are involved in this reaction. So it is a fair approximation to the negative feedback mechanism from Kholodenko, 2000.

inactiveRas-MKKK acting as a Product in a reaction in  
MAPK_osc Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
Neg_feedbackMAPK_osc
Accession No. : 27
MAPK
Pathway No. : 139
1
(uM^-1 s^-1)
0.009
(s^-1)
Kd(bf) = 0.009(uM)-Substrate
MAPK-PP
Ras-MKKKK

Product
  • inactiveRas-MKKK
  • From Kholodenko, 2000 Eur J Biochem 267 the Kd is 9 nM. We use a rather fast Kf of 1/sec/uM so that equilibrium is maintained.



    Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
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