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Molecule List for pathway Sos (Pathway Number 33) in Accession mkp1_feedback_effects (Accession Number 4)
| Default ordering is done according to Pathway Number. Table headers can be used for changing the default ordering.|
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The entries are grouped according to Pathway Number and are alternately color coded using and color.
|Buffered||Sum Total Of|
|1|| Grb2||Network||1||1000||No||- |
| || There is probably a lot of it in the cell: it is also known as Ash (abundant src homology protein). Also Waters et al JBC 271:30 18224 1996 say that only a small fraction of cellular Grb is precipitated out when SoS is precipitated. As most of the Sos seems to be associated with Grb2, it would seem like there is a lot of the latter. Say 1 uM. This would comfortably saturate the SoS.|
|2|| Sos||Network||0.1||1000||No||- |
| || I have tried using low (0.02 uM) initial concs, but these give a very flat response to EGF stim although the overall activation of Ras is not too bad. I am reverting to 0.1 because we expect a sharp initial response, followed by a decline. |
|3|| Sos*||Network||0||1000||No||- |
| || Phosphorylated form of SoS. Nominally this is an inactivation step mediated by MAPK, see Profiri and McCormick 1996 JBC 271(10):5871. I have not put this inactivation in this pathway so this molecule currently only represents a potential interaction point.|
|4|| Sos*.Grb2||Network||0||1000||No||- |
| || Inactive complex of Sos* with Grb2 due to phosphorylation of the Sos. [Porfiri E and McCormick F. (1996) J Biol Chem. 271(10):5871-5877]|
|5|| Sos.Grb2||Network||0||1000||No||- |
| || For simplicity I treat the activation of Sos as involving a single complex comprising Sos, Grb2 and Shc*. This is reasonably documented: Sasaoka et al 1994 JBC 269(51):32621-5 Chook et al JBC 1996 271(48):30472 |
|Database compilation and code copyright (C) 2005, Upinder S. Bhalla and NCBS/TIFR |
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