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Reaction Name | Pathway Name / Pathway No. | Kf | Kb | Kd | tau | Reagents |
1 | Ca-bind-CaNAB-Ca 2 | PP2B
Pathway No. 219 | 3.6 (uM^-2 s^-1) | 1 (s^-1) | Kd(af) = 0.527(uM) | - | Substrate: Ca Ca CaNAB-Ca2
Products: CaNAB-Ca4
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| This process is probably much more complicated and involves CaM. However, as I can't find detailed info I am bundling this into a single step. Based on Steemer and Klee pg 6863, the Kact is 0.5 uM. kf/kb = 1/(0.5 * 6e5)^2 = 1.11e-11 | 2 | Ca-bind-CaNAB | PP2B
Pathway No. 219 | 10008 (uM^-2 s^-1) | 1 (s^-1) | Kd(af) = 0.01(uM) | - | Substrate: CaNAB Ca Ca
Products: CaNAB-Ca2
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| going on the experience with CaM, we put the fast (high affinity) sites first. We only know (Stemmer and Klee) that the affinity is < 70 nM. Assuming 10 nM at first, we get kf = 2.78e-8, kb = 1. Try 20 nM. kf = 7e-9, kb = 1 | 3 | CaM-Ca2-bind-CaN AB | PP2B
Pathway No. 219 | 0.24 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 4.1667(uM) | - | Substrate: CaNAB-Ca4 CaM-TR2-Ca2
Products: CaMCa2-CANAB
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| Disabled. See notes for PP2B7.g | 4 | CaMCa3-bind-CaNA B | PP2B
Pathway No. 219 | 2.238 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.4468(uM) | - | Substrate: CaM-Ca3 CaNAB-Ca4
Products: CaMCa3-CaNAB
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5 | CaMCa4-bind-CaNA B | PP2B
Pathway No. 219 | 600 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 0.0017(uM) | - | Substrate: CaM-Ca4 CaNAB-Ca4
Products: CaMCa4-CaNAB
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