NCBS Home page
Accession List
Pathway List
Search
Authorized Users
Help
News archives

Accession Type:
Network
Ajay_Bhalla_
2007_PKM
Shared_Object_
Ajay_Bhalla_
2007_PKM
PKC
MAPK
Ras
 Molecule
 Enzyme
 Reaction
CaM
PKM

Enter a Search String

Special character and space not allowed in the query term. Search string should be at least 2 characters long.
Search in: Search for Match By

Enzyme List for pathway Ras (Pathway Number 372)

 Molecule Name/
Site Name
Km (uM) kcat (1/s)Ratio
(k2/k3)
Enzyme TypeSubstrate Product
1 Enzyme Activity:
basal_GEF_
activity

Enzyme Molecule:
inact-GEF
10.10140.024explicit E-S complexGDP-Ras
GTP-Ras
 
2 Enzyme Activity:
CaM-GEF-act-ras

Enzyme Molecule:
CaM-GEF
0.505050.24explicit E-S complexGDP-Ras
GTP-Ras
  Kinetics same as GEF-bg_act-ras, but as of March 17, 2006, the kcat is scaled from 0.02 to 0.2 to have a stronger Ca response for the direct MAPK input.
3 Enzyme Activity:
GAP-inact-ras

Enzyme Molecule:
GAP
1.0104104explicit E-S complexGTP-Ras
GDP-Ras
  From Eccleston et al JBC 268(36)pp27012-19 get Kd < 2uM, kcat - 10/sec From Martin et al Cell 63 843-849 1990 get Kd ~ 250 nM, kcat = 20/min I will go with the Eccleston figures as there are good error bars (10%). In general the values are reasonably close. k1 = 1.666e-3/sec, k2 = 1000/sec, k3 = 10/sec (note k3 is rate-limiting) 5 Nov 2002: Changed ratio term to 4 from 100. Now we have k1=8.25e-5; k2=40, k3=10. k3 is still rate-limiting.
4 Enzyme Activity:
GEF*-act-ras

Enzyme Molecule:
GEF*
0.505050.024explicit E-S complexGDP-Ras
GTP-Ras
  Kinetics same as GEF-bg-act-ras


Pathway Details   Molecule List  Enzyme List   Reaction List  


Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details.