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Pathway
SERCA
SERCA
 Molecule
 Reaction

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Reaction List for pathway SERCA (Pathway Number 187)

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reactions is not considered.
  Name KfKbKdtauSubstrateProduct
1 reac1kf
(s^-1)		0.35
(s^-1)		Keq = 1(uM)1.429secE2
E1
  kf and kb are the picked up from the Table-6 of literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
.
2 reac2kf
(uM^-1 s^-1)		120
(s^-1)		Kd(bf) = 2.4(uM)-E1
Ca
CaE1
  Kinetic constants for this step had substantial effects on the steady state EP level.
For SERCA2a alone the kf was 200uM
SERCA2a + Wild type Phospholamban kf was reduced to 100uM.
SERCA2a + L37A Phospholamban kf was reduced to 50um as specified by the author ( Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.).
kb in all the cases were maintained at 120/s.
3 reac3kf
(s^-1)		0.4
(s^-1)		Keq = 1(uM)1.25secCaE1
CaE1_prime
  kf and kb were taken form Table-6 of literature source
( Mahaney et al. ( 2000 Mar) Biophys J. 78(3) 1306-23.)
4 reac4kf
(uM^-1 s^-1)		3.2
(s^-1)		Kd(bf) = 0.04(uM)-CaE1_prime
Ca
Ca2E1_prime
  kf and kb were taken from the Table-6 of literature source
Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
5 reac5kf
(uM^-1 s^-1)		0
(s^-1)		--Ca2E1_prime
MgATP
CaE1_prime_ATP
  kf and kb were taken from the literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
6 reac6kf
(s^-1)		0
(uM^-1 s^-1)		--CaE1_prime_ATP
EP
ADP
  kf and kb were directly taken from the Table-6 of literature source Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
7 reac7kf
(s^-1)		0.7
(s^-1)		Keq = 2.3333(uM)1secEP
EPi
  kf and kb were taken from the literature source
Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
Some minor adjustments for steps 7 and 8 were required to prduce a precise simulation of the EP formation and Pi liberation time course
8 reac8kf
(s^-1)		0
(uM^-1 s^-1)		--EPi
pi
E2
  kf and kb were taken from the literature source
Mahaney et al. (2000 Mar) Biophys J. 78(3) 1306-23.
some minor adjustments for step7 and step8 were required to produce a precise simulation of the EP formation and Pi liberation time course as claimed by the author of above literature.


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