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Molecule Parameter List for PKA-active

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PKA-active participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	2	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
fig3_CaMKII	2	Network	Shared_Object_fig3_CaMKII,  CaMKII,  CaM,   PP1,  PP2B,  PKA,   AC
This is the model file for figure 3 from Bhalla US and Iyengar R. Science (1999) 283(5400):381-7. It is a model of the Ca activation of CaMKII and other CaM-activated enzymes. It includes the regulatory phosphatases PP1 and PP2B (Calcineurin) acting on CaMKII and also includes CaM-activated adenylyl cyclase and PKA in the synapse. Demonstration script files for generating the figures in the paper, including figure 3, are available here.

PKA-active acting as a Molecule in  fig3_CaMKII Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PKA-active	fig3_CaMKII Accession No. : 2	Shared_Object_ fig3_CaMKII Pathway No. : 12	0	1000	No

PKA-active acting as an Enzyme in  fig3_CaMKII Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	PKA-active / PKA-phosph-I1	fig3_CaMKII Accession No. : 2	Shared_Object_ fig3_CaMKII Pathway No. : 12	7.5	9	4	explicit E-S complex	Substrate I1 Product I1*
2	PKA-active / phosph-PDE	fig3_CaMKII Accession No. : 2	Shared_Object_ fig3_CaMKII Pathway No. : 12	7.5	9	4	explicit E-S complex	Substrate cAMP-PDE Product cAMP-PDE*

PKA-active acting as a Substrate in a reaction in  fig3_CaMKII Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
inhib-PKA	fig3_CaMKII Accession No. : 2	PKA Pathway No. : 17	60 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0167(uM)	-	Substrate PKA-active PKA-inhibitor Product inhibited-PKA

PKA-active acting as a Product in a reaction in  fig3_CaMKII Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Release-C1	fig3_CaMKII Accession No. : 2	PKA Pathway No. : 17	60 (s^-1)	18 (uM^-1 s^-1)	Kd(cb) = 0.3(uM)	-	Substrate R2C2-cAMP4 Product PKA-active R2C-cAMP4
	The complex starts to dissociate and release catalytic subunit C. This has to be fast, as the activation of PKA by cAMP is also fast.
2	Release-C2	fig3_CaMKII Accession No. : 2	PKA Pathway No. : 17	60 (s^-1)	18 (uM^-1 s^-1)	Kd(cb) = 0.3(uM)	-	Substrate R2C-cAMP4 Product PKA-active R2-cAMP4