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Molecule Parameter List for PKA-active

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

PKA-active participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	1	0	0	1	2

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
cAMP_pathway	25	Network	Shared_Object_cAMP_pathway,  PKA,  AC,   Gs
This is a model of the canonical cAMP signaling pathway: Ligand->Receptor->G-protein->Cyclase->cAMP->PKA. It also includes phosphodiesterases to balance out cAMP formation.Bhalla US Methods Enzymol. 2002;345:3-23

PKA-active acting as a Molecule in  cAMP_pathway Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
PKA-active	cAMP_pathway Accession No. : 25	Shared_Object_ cAMP_pathway Pathway No. : 134	0	1000	No

PKA-active acting as an Enzyme in  cAMP_pathway Network

Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
PKA-active / phosph-PDE	cAMP_pathway Accession No. : 25	Shared_Object_ cAMP_pathway Pathway No. : 134	7.5	9	4	explicit E-S complex	Substrate cAMP-PDE Product cAMP-PDE*
See Bramson et al CRC crit rev Biochem 15:2 93-124. The rates there are for peptide substrates and too fast. Scaled down by a factor of 3 as per Cohen et al FEBS Lett 76:182-86 (1977).

PKA-active acting as a Substrate in a reaction in  cAMP_pathway Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
inhib-PKA	cAMP_pathway Accession No. : 25	PKA Pathway No. : 135	60 (uM^-1 s^-1)	1 (s^-1)	Kd(bf) = 0.0167(uM)	-	Substrate PKA-active PKA-inhibitor Product inhibited-PKA
This has to be set to zero for matching the expts in vitro. In vivo we need to consider the inhibition though.

PKA-active acting as a Product in a reaction in  cAMP_pathway Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Release-C1	cAMP_pathway Accession No. : 25	PKA Pathway No. : 135	60 (s^-1)	18 (uM^-1 s^-1)	Kd(cb) = 0.3(uM)	-	Substrate cAMP4.R2C2 Product PKA-active cAMP4.R2C
	This has to be fast, as the activation of PKA by cAMP is also fast. kf was 10
2	Release-C2	cAMP_pathway Accession No. : 25	PKA Pathway No. : 135	60 (s^-1)	18 (uM^-1 s^-1)	Kd(cb) = 0.3(uM)	-	Substrate cAMP4.R2C Product PKA-active cAMP4.R2