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Molecule Parameter List for IP4(1345)

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

IP4(1345) participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	2	3	1	3

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NonOsc_Ca_ IP3metabolism	31	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  CaRegulation,   Gq,  PLCbeta,  134_dephos,   145_dephos,  IP4-system,  IHP-system,   1345_dephos
This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

IP4(1345) acting as a Molecule in  NonOsc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
IP4(1345)	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	1	1000	No
Inositol(1345)tetrakisphosphate

IP4(1345) acting as a Substrate for an Enzyme in  NonOsc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	IP_5pase1  / ip4_5pase	NonOsc_Ca_ IP3metabolism Accession No. : 31	145_dephos Pathway No. : 153	0.80003	0.09	4	explicit E-S complex	Substrate IP4(1345) Product IP3(134)
	Ins(1345)P4 5-phosphatase activity of InsP 5-phosphatase1 from Hansen et al, JBC 262; 1987 vmax for IP4 substrate is approx 0.1 times vmax for IP3
2	1345_3pase  / ip4_3pase	NonOsc_Ca_ IP3metabolism Accession No. : 31	1345_dephos Pathway No. : 156	0.400007	0.0012	4	explicit E-S complex	Substrate IP4(1345) Product IP3(145)
	Ins(1345)P4 3-phosphatase from Nogimori et al, JBC 266; 1991

IP4(1345) acting as a Product of an Enzyme in  NonOsc_Ca_IP3metabolism Network

	Enzyme Molecule / Enzyme Activity	Accession Name	Pathway Name	Km (uM)	kcat (s^-1)	Ratio	Enzyme Type	Reagents
1	IP3_3K  / ip3-3k	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	1.40001	3.0475	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Johanson et al, JBC 263; 1988 Original Vmax scaled up by 50% to obtain value at 37C, as enzyme assay was done at 30C Km increased from 0.2 to 1.4 as per various other reports (Shears Review, BiochemJ 260; 1989)
2	IP3_3K*1  / ip3-3k*1	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	1.40004	0.76187	4.00003	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Sim et al, JBC 265; 1990
3	IP3_3K*  / ip3-3k*	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	1.40001	3.0475	4	explicit E-S complex	Substrate IP3(145) Product IP4(1345)
	from Communi et al, EMBO J 16; 1997 In absence of CaM binding, activity same as that of non-phosphorylated enzyme

IP4(1345) acting as a Substrate in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
ip4(1345)_trp	NonOsc_Ca_ IP3metabolism Accession No. : 31	MIPP Pathway No. : 144	0.001 (s^-1)	1 (s^-1)	Not applicable**	-	Substrate IP4(1345) Product IP4(1345)_ER
Ins(1345)P4 ER-cytosol transport

** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration

IP4(1345) acting as a Product in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	3k-CaM*-off	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	45 (s^-1)	0.4148 (uM^-1 s^-1)	Kd(cb) = 0.0092(uM)	-	Substrate 3kCaM*_ip3_ cmplx Product IP3_3K_CaM* IP4(1345)
	Kf = Vmax for enzyme (Communi et al, EMBO J 16(8)) Vmax is such that enzyme activity is 9 fold above basal. Kb derived from Keq value when reaction free energy = -10 kJ/mol
2	3k-CaM-off	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP3-3K Pathway No. : 148	10.5 (s^-1)	0.0968 (uM^-1 s^-1)	Kd(cb) = 0.0092(uM)	-	Substrate 3kCaM_ip3_cmplx Product IP3_3K_CaM IP4(1345)
	Kf = Vmax for enzyme: Erneux et al, Biochem 214; 1993 Enzyme is 2-2.5 fold more active than basal enzyme. Kb derived from equilibrium conditions for dG = -10 kJ/mol
3	5kinase	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP4-system Pathway No. : 154	0.0701 (s^-1)	0.006 (uM^-1 s^-1)	Kd(cb) = 0.086(uM)	-	Substrate IP3-56Kcmplx Product IP3-56K_IP4-1K IP4(1345)
	Kf = 0.274 times Vmax of IP3 56-K as product ratio of Ins(1345)P4 : Ins(1346)P4 is 1 : 2.3-5 from Wilson and Majerus, JBC 271; 1996 Kb ascertained from dG calculations for equilibrium conditions., for a dG = -10 kJ/mol Also enzyme reversibility reported in Ho et al, Curr Biol 2002, 12: 1-20