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Molecule Parameter List for IP3-56Kcmplx

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

IP3-56Kcmplx participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
NonOsc_Ca_ IP3metabolism	31	Network	MIPP,  CaMKII,  CaM,   PKC,  IP3-3K,  CaRegulation,   Gq,  PLCbeta,  134_dephos,   145_dephos,  IP4-system,  IHP-system,   1345_dephos
This network models detailed metabolism of Ins(145)P3, integrated with GPCR mediated PLCbeta activation and Ca release by the InsP3 receptor in the neuron. It is similar to the NonOsc_Ca_IP3metab model (accession 23) except that some enzymes have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. These modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316.

IP3-56Kcmplx acting as a Molecule in  NonOsc_Ca_IP3metabolism Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
IP3-56Kcmplx	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP4-system Pathway No. : 154	0	1000	No
enzyme substrate complex of IP3 56-K and Ins(134)P3. Complex exclusively modeled as reaction generates ratio of products, and because 5-kinase is reversible due to large Ins(1345)P4 backflux

IP3-56Kcmplx acting as a Substrate in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	6kinase	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP4-system Pathway No. : 154	0.256 (s^-1)	0 (uM^-1 s^-1)	-	-	Substrate IP3-56Kcmplx Product IP3-56K_IP4-1K IP4(1346)
	Kf = Vmax for IP3 56-K from Yang and Shears, Biochem J 2000; 351:551-555 Enzyme reversibility reported in Ho et al, Curr Biol 2002, 12: 1-20. But backflow calculations do not show that Kb needs to be incorporated
2	5kinase	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP4-system Pathway No. : 154	0.0701 (s^-1)	0.006 (uM^-1 s^-1)	Kd(cb) = 0.086(uM)	-	Substrate IP3-56Kcmplx Product IP3-56K_IP4-1K IP4(1345)
	Kf = 0.274 times Vmax of IP3 56-K as product ratio of Ins(1345)P4 : Ins(1346)P4 is 1 : 2.3-5 from Wilson and Majerus, JBC 271; 1996 Kb ascertained from dG calculations for equilibrium conditions., for a dG = -10 kJ/mol Also enzyme reversibility reported in Ho et al, Curr Biol 2002, 12: 1-20

IP3-56Kcmplx acting as a Product in a reaction in  NonOsc_Ca_IP3metabolism Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
IP3-Kcmplx-on	NonOsc_Ca_ IP3metabolism Accession No. : 31	IP4-system Pathway No. : 154	4.2666 (uM^-1 s^-1)	1.024 (s^-1)	Kd(bf) = 0.24(uM)	-	Substrate IP3(134) IP3-56K_IP4-1K Product IP3-56Kcmplx
Kf and Kb are equivalent to k1 and k2 for InsP3 56-K, calculated from enzyme Km and Vmax: Yang and Shears, BiochemJ 2000, 351: 551-555