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Molecule Parameter List for PIP2 | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | Osc_Ca_
IP3metabolism | 32 | Network |
MIPP, CaMKII, CaM,
PKC, IP3-3K, Gq,
PLCbeta, 134_dephos, 145_dephos,
IP4-system, IHP-system, 1345_dephos,
CaRegulation, Othmer-Tang-model | | This network models an oscillatory calcium response to GPCR mediated PLCbeta activation, alongwith detailed InsP3 metabolism in the neuron. It is similar to the Osc_Ca_IP3metab model (accession 24) except that some enzymes in the InsP3 metabolism network have been modified to have reversible kinetics rather than Michaelis-Menten kinetics. The modified enzymes belong to the groups: IP4-system, IP3-3K, 145_dephos and 134_dephos. Mishra J, Bhalla US. Biophys J. 2002 Sep;83(3):1298-316. |
PIP2 acting as a Molecule in Osc_Ca_IP3metabolism Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | PIP2 | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 150 | 1000 | Yes | | PIP2 conc: Willars et al; JBC 273 (9) 27.2.98; pp 5037-5046 |
PIP2 acting as a Substrate for an Enzyme in Osc_Ca_IP3metabolism Network
| | Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | | 1 | PLC-Gq /
PLC-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 5 | 75 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | from Smrcka et al, 1991 Science 251: 804-807 | | 2 | PLC-Ca /
PLC-Ca | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 19.8408 | 10 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | From Sternweis et al Phil Trans R Soc Lond 1992, also matched by Homma et al. k1 = 1.5e-5, now 4.2e-6 k2 = 70/sec; now 40/sec k3 = 17.5/sec; now 10/sec Note that the wording in Sternweis et al is ambiguous re the Km. Also Smrcka et al; Science 251, 15.2.1991, pp804-807 | | 3 | PLC-Ca-Gq /
PLCb-Ca-Gq | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 4.99994 | 160 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | Km: Sternweis et al, Phil Trans R Soc Lond 1992 Vmax: Smrcka et al, Science 1991 | | 4 | PLC /
PLC | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 19.9994 | 2.5 | 4 | explicit E-S complex | Substrate
PIP2
Product
DAG
IP3(145)
| | | Smrcka et al; Science 251, 15.2.1991, pp804-807 |
PIP2 acting as a Substrate in a reaction in Osc_Ca_IP3metabolism Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | basal | Osc_Ca_
IP3metabolism
Accession No. : 32 | PLCbeta
Pathway No. : 164 | 0.003
(s^-1) | 0
(uM^-1 s^-1) | - | - | Substrate
PIP2
Product
DAG
IP3(145)
| | accounts for other PLC isoforms that contribute to basal levels of IP3 |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
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