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Molecule Parameter List for E0* | The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by  color. | | Statistics |
Accession and Pathway Details | |
| Accession Name | Accession No. | Accession Type | Pathway Link | MAPK_Asthagiri_
enz_fb | 46 | Pathway |
MAPK_Asthagiri_enz_fb | This is a complex model to examine mechanisms that govern MAPK pathway dynamics in Chinese hamster ovary (CHO) cell lines, particularly the role of enzyme targeted negative feedback mechanism in generating downstream signal adaptation. This model simulates the results as per the simulation conditions specified for figure 7B of the paper by Asthagiri AR and Lauffenburger DA. Biotechnol Prog. (2001) 17(2):227-39.
However, there is some discrepancy with the outputs shown in Figure 7B. |
E0* acting as a Molecule in MAPK_Asthagiri_enz_fb Network
| Name | Accession Name | Pathway Name | Initial Conc.
(uM) | Volume
(fL) | Buffered | | E0* | MAPK_Asthagiri_
enz_fb
Accession No. : 46 | MAPK_Asthagiri_
enz_fb
Pathway No. : 194 | 0 | 390 | No | | Active form of enzyme from Asthagiri and Lauffenburger, 2001 |
E0* acting as an Enzyme in MAPK_Asthagiri_enz_fb Network
Enzyme Molecule /
Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents | E0* /
E1_activ
| MAPK_Asthagiri_
enz_fb
Accession No. : 46 | MAPK_Asthagiri_
enz_fb
Pathway No. : 194 | 0.0600001 | 0.1 | 5 | explicit E-S complex | Substrate
E1
Product
E1*
| | k1+ =6*10^8 M-1 min-1, k1- =30 min-1, kcat,1 =6 min-1 and km caluculated to 0.06 from Asthagiri and Lauffenburger 2001. |
E0* acting as a Product in a reaction in MAPK_Asthagiri_enz_fb Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated.
Kd for higher order reaction are not consider. |
| | Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents | | 1 | C*A1-bind-A2 | MAPK_Asthagiri_
enz_fb
Accession No. : 46 | MAPK_Asthagiri_
enz_fb
Pathway No. : 194 | 5.0001
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.1(uM) | - | Substrate
A2
C*A1
Product
E0*
| | | kf2 and kr2 are 3*10^8 M-1 min-1 and 0.5/sec from Asthagiri and Lauffenburger , 20001. | | 2 | C*-bind-A1A2 | MAPK_Asthagiri_
enz_fb
Accession No. : 46 | MAPK_Asthagiri_
enz_fb
Pathway No. : 194 | 5.0001
(uM^-1 s^-1) | 0.5
(s^-1) | Kd(bf) = 0.1(uM) | - | Substrate
A1A2
C*
Product
E0*
| | | Kf12 and Kr12 are 3*10^8 M-1 min-1 and 0.5/sec from Asthagiri and Lauffenburger 2001. |
| Database compilation and code copyright (C) 2022, Upinder S. Bhalla and NCBS/TIFR
This Copyright is applied to ensure that the contents of this database remain freely available. Please see FAQ for details. |
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