| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
1 | Complexing | Chemotaxis Accession No. : 57 | Chemotaxis Pathway No. : 228 | 0.1 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 10.0003(uM) | - | Substrate CheW Tar
Product TW
|
| Tar and CheW complex formation Kf = 1 * 10e5 /sec/M = 0.1 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 3 |
2 | Binding | Chemotaxis Accession No. : 57 | Chemotaxis Pathway No. : 228 | 0.001 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 1000.028(uM) | - | Substrate Ni Tar
Product Tn
|
| Binding of Tar and Ni Kf = 1 * 10e+03 /sec/M = 0.001 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 2 |
3 | Binding[1] | Chemotaxis Accession No. : 57 | Chemotaxis Pathway No. : 228 | 12.8 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 0.7813(uM) | - | Substrate Aspartate Tar
Product TA
|
| Binding of Aspartate with Tar Kd = 0.78 uM Dunten and Koshland, 1991 Kf = 1.28e+06 /sec/M = 1.28 /sec/uM here scaled by 5 As per Reaction 0 in 1REACT.BCT provided by Matthew Levin Kf = 1.0e+06 /sec/M = 1 /sec/uM; Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 1 |
4 | Binding[3] | Chemotaxis Accession No. : 57 | Chemotaxis Pathway No. : 228 | 0.05 (uM^-1 s^-1) | 1 (s^-1) | Kd(bf) = 19.9999(uM) | - | Substrate CheA Tar
Product TA
|
| Binding of T and CheA Kf = 0.05 /sec/uM as per Reaction 3 in 1REACT.BCT Kf = 1*10e+04 /sec/M = 0.01 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 4 |
5 | Binding[9] | Chemotaxis Accession No. : 57 | Chemotaxis Pathway No. : 228 | 4 (uM^-1 s^-1) | 10 (s^-1) | Kd(bf) = 2.5(uM) | - | Substrate Tar WA
Product TWA
|
| Binding of Tar and CheW-CheA complex Kd = 2 uM and therefore Kf also suggested as 0.5 /sec/uM Kf = 4e+05 /sec/M = 0.4 /sec/uM Kb = 1 /sec Bray et al 1993, Mol.Biol.Cell 4: 469-482 Table 3 pp.476 Reaction Scheme 8 Rates used here are 10 times that stated, to allow for faster time courses seen during the drop of bias on removal of stimulus. |