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Molecule Parameter List for GDP.Ran-nuclear

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
GDP.Ran-nuclear participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000012

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
RanGTPase70Network
Shared_Object_RanGTPase,  Nucleus,  Cytoplasm 
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

GDP.Ran-nuclear acting as a Molecule in  RanGTPase Network
NameAccession NamePathway NameInitial Conc.
(uM)		Volume
(fL)		Buffered
GDP.Ran-nuclearRanGTPase
Accession No. : 70		Nucleus
Pathway No. : 305		012000No
nuclear-GDP.Ran Nuclear fraction of GDP bound Ran Approximately 71% of RanGDP in the nucleus is bound Nuclear volume = 1.2 pL = 1.2e-15 m^3 Gorlich D et al (2003) EMBO J 22(5):1088-1100

GDP.Ran-nuclear acting as a Substrate in a reaction in  RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
NameAccession NamePathway NameKfKbKdtauReagents
RCC1_bindingRanGTPase
Accession No. : 70		Nucleus
Pathway No. : 305		74.0006
(uM^-1 s^-1)		55
(s^-1)		Kd(bf) = 0.7432(uM)-Substrate
GDP.Ran-nuclear
RCC1

Product
RCC1-GDP.Ran
Binding of GDP-Ran complex with RCC1 Kf = 7.4e+07 /M/sec = 7.4e+01 /uM/sec Kb = 55 /sec Christian Klebe et al (1995) Biochemistry 34:12543-12552. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100

GDP.Ran-nuclear acting as a Product in a reaction in  RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1
  • NTF2_
    dissociation[1]
    		• RanGTPase
    Accession No. : 70    		Nucleus
    Pathway No. : 305    		0.48
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-Substrate
  • nuclear-NTF2-GDP
    .Ran

    Product
    GDP.Ran-nuclear
    NTF2
    •   Approximation of reaction rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    2
  • NTF2_
    dissociation
    		• RanGTPase
    Accession No. : 70    		Cytoplasm
    Pathway No. : 306    		0.48
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-Substrate
    NTF2-GDP.Ran

    Product
    GDP.Ran-nuclear
    NTF2
      Approximation of rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    ** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration


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