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Molecule Parameter List for GTP.Ran-cytoplasmic | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by  color. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| Statistics | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| GTP.Ran-cytoplasmic participated as | Molecule | Sum total of | Enzyme | Substrate of an enzyme | Product of an enzyme | Substrate in Reaction | Product in Reaction |
| No. of occurrences | 1 | 0 | 0 | 1 | 0 | 1 | 1 |
Accession and Pathway Details |
| Accession Name | Accession No. | Accession Type | Pathway Link |
| RanGTPase | 70 | Network | Shared_Object_RanGTPase, Nucleus, Cytoplasm |
| This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs. | |||
GTP.Ran-cytoplasmic acting as a Molecule in RanGTPase Network
| Name | Accession Name | Pathway Name | Initial Conc. (uM) | Volume (fL) | Buffered | |
| GTP.Ran-cytoplasmic | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 0 | 18000 | No | |
| cytoplasmic-GTP.Ran Cytoplasmic fraction of Ran-GTP complex Cytoplasmic volume = 1.8 pL = 1.8e-16m^3 Gorlich D et al (2003) EMBO J 22(5):1088-1100 | ||||||
GTP.Ran-cytoplasmic acting as a Substrate for an Enzyme in RanGTPase Network
| Enzyme Molecule / Enzyme Activity | Accession Name | Pathway Name | Km (uM) | kcat (s^-1) | Ratio | Enzyme Type | Reagents |
| RanGAP / RanGAP[1] | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 0.699998 | 10.6 | 4 | explicit E-S complex | Substrate mic Product mic |
| RanGAP reaction on free RanGTP kcat = 10.6 /sec Km = 0.7 uM at 25oC Table 1. Gorlich D et al (2003) EMBO J 22(5):1088-1100 | |||||||
GTP.Ran-cytoplasmic acting as a Substrate in a reaction in RanGTPase Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
| RanGTP_binding | RanGTPase Accession No. : 70 | Cytoplasm Pathway No. : 306 | 18.0004 (uM^-1 s^-1) | 0.0004 (s^-1) | Kd(bf) = 0(uM) | - | Substrate mic RanBP1 Product RanBP1.RanGTP |
GTP.Ran-cytoplasmic acting as a Product in a reaction in RanGTPase Network
| Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider. |
| Name | Accession Name | Pathway Name | Kf | Kb | Kd | tau | Reagents |
transfer | RanGTPase Accession No. : 70 | RanGTPase Pathway No. : 304 | 0.08 (s^-1) | 1.3 (s^-1) | Not applicable** | - | Substrate GTP.Ran-nuclear Product mic |
| Approximation to achieve Cytoplasmic transfer as per kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100 | |||||||