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Molecule Parameter List for NTF2-GDP.Ran

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network.
The text color of a molecule is highlighted by color.
Statistics
NTF2-GDP.Ran participated asMoleculeSum total ofEnzymeSubstrate of an enzymeProduct of an enzymeSubstrate in ReactionProduct in Reaction
No. of occurrences1000021

Accession and Pathway Details
Accession NameAccession No.Accession TypePathway Link
RanGTPase70Network
Shared_Object_RanGTPase,  Nucleus,  Cytoplasm 
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

NTF2-GDP.Ran acting as a Molecule in  RanGTPase Network
NameAccession NamePathway NameInitial Conc.
(uM)		Volume
(fL)		Buffered
NTF2-GDP.RanRanGTPase
Accession No. : 70		Cytoplasm
Pathway No. : 306		018000No
NTF2-GDP.Ran Cytoplasmic fraction of GDP bound Ran complexed with NTF2 Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2 Gorlich D et al (2003) EMBO J 22(5):1088-1100 Initial cytoplasmic GDP.Ran concentration = 5 uM Table II footnote

NTF2-GDP.Ran acting as a Substrate in a reaction in  RanGTPase Network
Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
 NameAccession NamePathway NameKfKbKdtauReagents
1
  • Nucleoplasmic_
    transfer
    		• RanGTPase
    Accession No. : 70
  • Shared_Object_
    RanGTPase
    Pathway No. : 304
    		• 10000
    (s^-1)    		0.1
    (s^-1)    		Not applicable**-Substrate
    NTF2-GDP.Ran

    Product
  • nuclear-NTF2-GDP
    .Ran
    •   Approximation to achieve Nucleoplasmic transfer as per kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    2
  • NTF2_
    dissociation
    		• RanGTPase
    Accession No. : 70    		Cytoplasm
    Pathway No. : 306    		0.48
    (s^-1)    		0
    (#^-1 s^-1)    		Not applicable**-Substrate
    NTF2-GDP.Ran

    Product
    GDP.Ran-nuclear
    NTF2
      Approximation of rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
    ** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration

    NTF2-GDP.Ran acting as a Product in a reaction in      RanGTPase Network
    Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.
    NameAccession NamePathway NameKfKbKdtauReagents
    NTF2_bindingRanGTPase
    Accession No. : 70    		Cytoplasm
    Pathway No. : 306    		1
    (uM^-1 s^-1)    		0.1
    (s^-1)    		Kd(bf) = 0.1(uM)-Substrate
  • GDP.Ran-cytoplas
    mic
    NTF2

    Product
    NTF2-GDP.Ran
    • Binding of NTF2 to cytoplasmic fraction of GDP.Ran Kd = 100 nM Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. call /kinetics/Cytoplasm/RanGTP_binding/notes LOAD Binding of cytoplasmic fraction of RanGTP to RanBP1 Kf = 3.0e+05 /M/sec = 0.3 /uM/sec Kb = 4.0e-04 /sec Carolina I. Villa Braslavsky et al (2000) Biochemistry 39(38):11629-11639. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100


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