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Molecule Parameter List for NTF2-GDP.Ran

The statistics table lists the distribution of a molecule acting either as a substrate, product, enzyme or as a molecule within the network. The text color of a molecule is highlighted by color.

Statistics

NTF2-GDP.Ran participated as	Molecule	Sum total of	Enzyme	Substrate of an enzyme	Product of an enzyme	Substrate in Reaction	Product in Reaction
No. of occurrences	1	0	0	0	0	2	1

Accession and Pathway Details

Accession Name	Accession No.	Accession Type	Pathway Link
RanGTPase	70	Network	Shared_Object_RanGTPase,  Nucleus,  Cytoplasm
This model represents a concentration gradient of RanGTP across the nuclear envelope. This gradient is generated by distribution of regulators of RanGTPase. We have taken a log linear plot of graphs generated by GENESIS and compared with the experimental graphs.

NTF2-GDP.Ran acting as a Molecule in  RanGTPase Network

Name	Accession Name	Pathway Name	Initial Conc. (uM)	Volume (fL)	Buffered
NTF2-GDP.Ran	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	0	18000	No
NTF2-GDP.Ran Cytoplasmic fraction of GDP bound Ran complexed with NTF2 Nuclear volume = 1.2 pL = 1.2e-15 m^3 Footnote for Table 2 Gorlich D et al (2003) EMBO J 22(5):1088-1100 Initial cytoplasmic GDP.Ran concentration = 5 uM Table II footnote

NTF2-GDP.Ran acting as a Substrate in a reaction in  RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

	Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
1	Nucleoplasmic_ transfer	RanGTPase Accession No. : 70	Shared_Object_ RanGTPase Pathway No. : 304	10000 (s^-1)	0.1 (s^-1)	Not applicable**	-	Substrate NTF2-GDP.Ran Product nuclear-NTF2-GDP .Ran
	Approximation to achieve Nucleoplasmic transfer as per kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100
2	NTF2_ dissociation	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	0.48 (s^-1)	0 (#^-1 s^-1)	Not applicable**	-	Substrate NTF2-GDP.Ran Product GDP.Ran-nuclear NTF2
	Approximation of rates to match the kinetics described by Gorlich D et al (2003) EMBO J 22(5):1088-1100

** This is a trasport reation between compartments of different volumes. Therefore Kd is not applicable. Please Note Kf, Kb units are in number of molecules instead of concentration

NTF2-GDP.Ran acting as a Product in a reaction in  RanGTPase Network

Kd is calculated only for second order reactions, like nA+nB <->nC or nA<->nC+nD, where n is number and A,B,C,D are molecules, where as for first order reactions Keq is calculated. Kd for higher order reaction are not consider.

Name	Accession Name	Pathway Name	Kf	Kb	Kd	tau	Reagents
NTF2_binding	RanGTPase Accession No. : 70	Cytoplasm Pathway No. : 306	1 (uM^-1 s^-1)	0.1 (s^-1)	Kd(bf) = 0.1(uM)	-	Substrate GDP.Ran-cytoplas mic NTF2 Product NTF2-GDP.Ran
Binding of NTF2 to cytoplasmic fraction of GDP.Ran Kd = 100 nM Catherine Chaillan-Huntington et al (2000) J.Biol.Chem. 275(8):5874-5879. call /kinetics/Cytoplasm/RanGTP_binding/notes LOAD Binding of cytoplasmic fraction of RanGTP to RanBP1 Kf = 3.0e+05 /M/sec = 0.3 /uM/sec Kb = 4.0e-04 /sec Carolina I. Villa Braslavsky et al (2000) Biochemistry 39(38):11629-11639. Supplementary Table A Gorlich D et al (2003) EMBO J 22(5):1088-1100